Thermal Stability (DSC, DSF) Analysis of Peptide Drugs

Peptide drugs are bioactive molecules formed by the connection of multiple amino acids through peptide bonds, most of which originate from endogenous peptides or natural peptides. Therefore, they have no side effects or very minor side effects for the human body. Compared with protein-based drugs, peptide drugs also have advantages like better stability, higher purity, lower production cost, and lower immunogenicity. Coupled with the rapid development and maturity of peptide synthesis technology, peptide drugs have become the focus of biopharmaceutical research in recent years. Currently, peptide drugs are widely used in the diagnosis and treatment of tumors, autoimmune diseases, certain cardiovascular diseases, diabetes, and more. They show broad application prospects.

 

Thermal stability refers to the ability of a substance to resist irreversible changes (usually by resisting decomposition or polymerization) in its chemical or physical structure at relatively high temperatures. The structure of proteins/peptides is a key factor in determining their function. For proteins/peptides that cannot withstand high temperatures, their high-level structure will be destroyed as the heat increases, causing the proteins/peptides to unfold and become inactive. Therefore, analyzing the thermal stability of protein biological products is one of the main contents in their quality control. Differential Scanning Calorimetry (DSC) and Differential Scanning Fluorimetry (DSF) are commonly used techniques to characterize thermal stability. They are used to evaluate the thermodynamic property changes of proteins/peptides during heating, but can also be used in ligand interaction studies, rapid optimization of purification and production conditions, and quick determination of the optimum conditions for liquid formulations, etc.

 

MtoZ Biolabs provides you with one-stop peptide drug thermal stability analysis services. You can choose different methods according to your needs to analyze the stability of peptide drugs at different temperatures, identify and select the most stable peptides during the development of biological therapeutic drugs. This provides important guidance for the development, manufacturing, and quality control of peptide drugs.