Tomato Fruit Tandem Mass Spectrometry Peptide Sequencing

Tomatoes, as one of the vegetable crops with the largest planting area and consumption in the world, their fruits are not only delicious, but also rich in nutrients and bioactive substances. All of these are closely related to the expression, modification and interaction of proteins and peptides in tomato fruits. Tandem mass spectrometry sequencing can provide us detailed information about proteins/peptides, revealing the interactions, functions, and structures of proteins, which is particularly important for studying the ripening, quality, flavor and disease resistance of tomato fruits.

 

Principle of Tandem Mass Spectrometry Peptide Sequencing

Tandem mass spectrometry (MS/MS or tandem MS) sequencing analyzes peptides using a two-stage mass analyzer through specific isolation, fragmentation, and detection processes. In the first stage of mass analysis, specific precursor ions (i.e., unfragmented peptide ions) are isolated. Subsequently, these precursor ions collide with gas molecules in the collision chamber, causing ion fragmentation. Finally, these fragments are further analyzed in the second stage of mass analysis, providing detailed information about the original peptide sequence.

 

Tomato Peptide Tandem Mass Spectrometry Sequencing Technology of  MtoZ Biolabs

MtoZ Biolabs uses Thermo's latest Obitrap Fusion Lumos mass spectrometer in combination with Nano-LC nanoscale chromatography technology to provide mass spectrometry-based tomato peptide sequence analysis services. The Obitrap Fusion Lumos mass spectrometer has the highest resolution and sensitivity, ensuring the sensitivity of low abundance peptide fragment identification; meanwhile, it adopts a combination of HCD and ETD modes during the fragmentation of peptide segments to ensure the integrity of peptide fragment fragments, maximizing the accuracy of peptide sequencing results.

 

Application Areas

1. Proteomic Research

This is the most direct application area of tandem mass spectrometry technology. By analyzing the protein composition in tomato fruits, scientists can identify and quantify the proteins in tomato fruits and understand their functions and interactions. This helps reveal the molecular mechanisms of tomato fruit growth and development, maturation, and responses to environmental factors.

 

2. Variety Improvement and Genetic Research

By analyzing protein differences in different tomato varieties or genetically modified tomatoes, scientists can identify proteins or genes associated with specific traits, such as disease resistance, fruit size, color, and nutritional components. This provides molecular level information and tools for tomato breeding and genetic improvement.

 

3. Nutrition and Metabolism Research

Through the analysis of proteins in tomato fruits, the biosynthesis pathways of its nutritional components can be deeply understood, including vitamins, antioxidants, and other health-beneficial secondary metabolites. This helps to develop healthier, higher nutritional value tomato varieties.

 

4. Research on Coping With Environmental Stress

Environmental stresses such as drought, salt stress, and temperature changes have a significant impact on the growth and yield of tomato fruits. Through tandem mass spectrometry peptide sequencing, proteins with expression changes under specific environmental stress can be identified, revealing the molecular mechanisms of tomatoes' response to adversity, and providing a basis for breeding varieties with stronger resistance.

 

MtoZ Biolabs has advanced equipment and an experienced expert team, committed to providing scholars and researchers with accurate, fast, and efficient services. We understand the unique needs of each customer and tailor solutions for them. Feel free to consult for more details!