Total Trans Fatty Acid Analysis Service

Fatty acid (FAs) are the main components of lipid, and the physical, chemical, and physiological properties of lipids mainly depend on their fatty acid composition. The composition of fatty acid is determined as the methyl esters by gas chromatography (GC). Trans-fatty acids are a type of unsaturated fat that occur in small amounts in nature, but have been widely industrially produced from vegetable fats since the 1950s for use in margarine, packaged baked goods, snack foods, and frying fast food. It has been proven that transfat have been associated with an increased risk of coronary heart disease (the leading cause of death in Western countries).

 

GC-MS provides a multitude of methods for the qualitative characterization of FAs. For example, using 70 eV EI ionization, analysis of pyrrolidides, dimethyloxazolines, pyridinyl derivatives, pentafluorodimethylsilyl derivatives, and trimethylsilyl derivatives of FAs can be performed for structural determination. The EI ionization of these less common FA derivatives can produce different fragmentation patterns that help locate the branching positions and unsaturation sites of FAs. The simplest method for quantitative analysis of methylated fatty acids by EI-MS can be performed by monitoring a series of mass-to-charge ratio (m/z) values covering the expected fragments of the analyte and then determining the content based on the integration of the chromatographic peaks in the total ion chromatogram (TIC).

 

MtoZ Biolabs provides reliable, fast, and cost-effective total trans-fatty acid analysis services based on stable, reproducible, and sensitive systems for separation, characterization, identification, and quantitative analysis of trans-fatty acid. Free consultation is available!