Western Blot Protein Quantification Service

Western blot protein quantification is a method that employs western blot technology to quantitatively analyze target proteins, enabling precise measurement of their expression levels in samples and providing critical data for studying protein function, regulatory mechanisms, and disease progression. Unlike traditional qualitative analysis, western blot protein quantification reveal changes in protein expression under varying experimental conditions, aiding researchers in better understanding the regulation of key proteins in biological processes.

 

Leveraging advanced equipment and a professional technical team, MtoZ Biolabs offers precise western blot protein quantification service. We are committed to providing reliable, reproducible data to support your research and development needs.

 

Technical Principles

The western blot protein quantification service integrates protein separation, antibody specificity, and sensitive signal detection to ensure accurate quantification. SDS-PAGE is first used to separate proteins based on molecular weight, generating well-defined protein bands for subsequent analysis.

 

Following separation, proteins are transferred onto PVDF or nitrocellulose membranes. Depending on experimental needs, MtoZ Biolabs employs either wet transfer or semi-dry transfer methods. Wet transfer offers high efficiency and protein integrity, making it ideal for large proteins, but requires more time and buffer. Semi-dry transfer, by contrast, is faster and more resource-efficient, suitable for smaller proteins or time-sensitive experiments. The optimal method is selected to maximize transfer efficiency and ensure data quality.

 

Blocking agents such as skimmed milk or BSA are then applied to reduce background noise, followed by specific primary antibodies to bind the target protein. Secondary antibodies conjugated with enzymes (e.g., HRP) or fluorophores amplify the signal, which is detected using chemiluminescence or fluorescence imaging systems. Professional software analyzes signal intensity, delivering reproducible and high-quality quantitative results.

 



For added reliability, MtoZ Biolabs can combine western blot with mass spectrometry (MS) to identify proteins and analyze post-translational modifications such as phosphorylation and acetylation. This integrated approach enhances result accuracy and supports complex protein studies with comprehensive data.

 

Services at MtoZ Biolabs

MtoZ Biolabs’ western blot protein quantification service includes the following offerings:

1. Target Protein Quantitative Analysis: Provides high-precision relative or absolute quantification data using reference proteins for normalization.

2. Multi-target Detection: Enables simultaneous analysis of multiple targets on a single membrane to address complex experimental requirements.

3. Protein Modification Analysis: Conducts quantitative studies on post-translational modifications (e.g., phosphorylation, acetylation) to understand protein regulation.

4. Mass Spectrometry Coupled Validation: Utilizes MS to identify proteins and analyze their modifications, enhancing the reliability of western blot data.

5. Specific Antibody Screening: Selects and validates high-quality antibodies to ensure the success and accuracy of experiments.

6. Customized Solutions: Designs and optimizes experimental protocols tailored to specific research needs, offering personalized analysis services.

 

Why Choose MtoZ Biolabs?

MtoZ Biolabs’ western blot protein quantification service offers several key advantages:

• Unmatched Sensitivity and Specificity: Utilizes high-quality antibodies and optimized protocols to ensure the precise detection of low-abundance proteins.
• Versatile Applications: Compatible with a wide range of sample types, including cells, tissues, serum, and purified proteins, catering to diverse research needs.
•  Streamlined Multiplexing: Supports multiple detection methods such as chemiluminescence and fluorescence, allowing for efficient and comprehensive analysis.
• Customized Solutions: Offers flexible and tailored experimental protocols to meet specific research objectives and requirements.
• Reliable Data Quality: Implements stringent quality control measures and thorough data analysis to ensure the reliability and reproducibility of results.

 

Sample Submission Suggestions

To ensure the accuracy and reliability of our western blot protein quantification service, MtoZ Biolabs recommends submitting the following types of samples:

• Cell Samples: Total protein concentration of 1–2 mg/mL, with a volume of at least 50 µL.
• Tissue Samples: Fresh or flash-frozen tissues, prepared to a concentration of 1–2 mg/mL.
• Serum/Plasma: A minimum volume of 200 µL.
• Purified Proteins: Concentration of ≥1 mg/mL and purity of ≥90%.
• Special Samples: For unique or specialized sample types, please consult with our technical team in advance.

When submitting samples, please provide comprehensive information, including sample type, source, and processing methods. MtoZ Biolabs will tailor the experimental protocol based on the specific characteristics of your samples to ensure the delivery of high-quality quantitative data.

 

Applications

MtoZ Biolabs’ western blot protein quantification service is extensively applied in various research areas, including:

• Protein Expression Analysis: Evaluates the expression levels of target proteins under different biological or experimental conditions to understand protein dynamics.
• Drug Development and Mechanism Studies: Investigates how pharmaceutical compounds regulate protein expression and function, aiding in the development of new therapeutics.
• Disease Mechanism Research: Explores the correlation between protein expression levels and the onset or progression of diseases, contributing to the understanding of disease pathology.
• Biomarker Discovery and Validation: Quantitatively validates potential biomarkers for diagnostic or therapeutic applications, enhancing their reliability and applicability.
• Signal Pathway Analysis: Identifies and quantifies key signaling molecules to map and elucidate intracellular signal transduction pathways, advancing the knowledge of cellular mechanisms.

 

MtoZ Biolabs provides professional western blot protein quantification service designed to support and accelerate your research and development projects. Contact our technical team today to learn more about how we can assist you in achieving breakthrough scientific advancements!