What Is Used to Identify the Modification of a Specific Protein
Specific protein modifications can be identified using a variety of methodologies, including:
1. Mass Spectrometry Fingerprinting
This is a robust technique employed to identify proteins and their modifications. By examining the mass and distribution of proteins or peptide fragments, specific post-translational modifications such as phosphorylation, glycosylation, and ubiquitination can be accurately identified.
2. Western Blot
This technique uses specific antibodies to detect particular protein modifications. For example, antibodies targeting phosphorylated sites are used to study protein phosphorylation.
3. Enzyme-Linked Immunosorbent Assay (ELISA)
Comparable to Western Blot but more suitable for quantitative analysis.
4. Co-Immunoprecipitation (Co-IP)
This method is used to investigate protein-protein interactions and can indirectly identify protein modifications.
5. Fluorescence Resonance Energy Transfer (FRET) or Two-Photon Microscopy
These are used to explore protein dynamics and modifications within living cells.
6. Chromatin Immunoprecipitation (ChIP)
This is applied to study DNA-associated protein modifications, such as histone modifications.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
How to order?
