β-Hydroxybutyric Acid Analysis Service
- Diagnose and monitor diabetes, ketosis, and other metabolic diseases by detecting BHB levels.
- Study the role of BHB in energy metabolism and metabolic regulation.
- Evaluate changes in BHB during exercise to optimize training and nutrition strategies.
- Explore the biological activities of BHB and its potential therapeutic applications in disease treatment.
β-Hydroxybutyric acid, also known as 3-hydroxybutyric acid or BHB, is a vital biological molecule involved in energy metabolism and physiological functions. It is a type of ketone body synthesized mainly in the liver through fatty acid oxidation, serving as an essential energy source during fasting, diabetes, or prolonged exercise. The levels of BHB in blood, urine, and other biological samples indicate an individual's metabolic state, making it crucial in clinical diagnostics, metabolic research, and sports science. Additionally, BHB exhibits neuroprotective, anti-inflammatory, and antioxidant properties, indicating its potential therapeutic applications in various diseases.
Figure 1. Molecular Structure of β-Hydroxybutyric Acid
MtoZ Biolabs offers β-Hydroxybutyric Acid Analysis Service using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). This advanced method combines the separation capabilities of UPLC with the detection power of MS, ensuring high sensitivity and specificity in BHB detection.
Analysis Workflow
Service Advantages
1. High Sensitivity and Precision
UPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of BHB.
2. Rapid and Efficient
Optimized sample preparation and fast analysis ensure quick and efficient processing.
3. Wide Applicability
Suitable for various biological samples, including blood, urine, and tissues.
4. Accurate and Reliable Data
Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.
Sample Submission Requirements
1. Sample Types
Biological Fluids: Serum, plasma, urine, bile, bile acids
Animal Tissues: Cells, liver, brain, and other animal tissues, as well as feces
Other Samples: Plants, yeast, microorganisms
2. Sample Volume
At least 200 μL of liquid sample or 200 mg of solid sample.
3. Sample Transportation
For shipping, it is recommended to use an adequate amount of dry ice, considering that 3-4 kg of dry ice sublimates in one day. It is advisable to use larger blocks of dry ice as they sublimate more slowly. Ensure samples are sealed in a foam box and opt for the fastest possible shipping method to minimize sample degradation during transport.
Applications
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on β-Hydroxybutyric Acid
4. Raw Data
5. Custom Analysis Report
Our β-Hydroxybutyric Acid Analysis Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.
How to order?
