γ-Aminobutyric Acid Analysis Service
- Liquid Chromatography-Mass Spectrometry (LC-MS)
- Gas Chromatography-Mass Spectrometry (GC-MS)
- Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
γ-Aminobutyric Acid (GABA) is a crucial neurotransmitter widely present in the central nervous system. It plays a significant role in regulating neuronal excitability, relieving stress, and promoting sleep. GABA is derived from the amino acid glutamate. Through the catalytic action of glutamate decarboxylase (GAD), glutamate loses a carboxyl group (COOH) to form GABA. The metabolism of GABA involves multiple enzymes and pathways, including its conversion to succinic semialdehyde by GABA transaminase (GABA-T), which then enters the tricarboxylic acid (TCA) cycle.
Abnormal GABA metabolism is associated with various neurological disorders, such as epilepsy, anxiety, and depression. Therefore, analyzing GABA and its metabolites is vital for understanding their functions and mechanisms within the body. Accurate and rapid detection of GABA levels is invaluable for disease research, drug development, and neuroscience.
Figure 1. Molecular Structure of GABA
MtoZ Biolabs utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to conduct detailed quantitative and qualitative analyses of GABA and its metabolites. This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of GABA in complex biological matrices.
Analysis Workflow
Experimental Instruments
Service Advantages
Sample Submission Requirements
Sample Types
Serum, plasma, urine, tissue homogenates, and other biological samples.
Sample Volume
At least 200 μL of liquid sample or 200 mg of tissue sample.
Applications
Neurological Disease Research: Investigating the role of GABA metabolism in epilepsy, anxiety, and depression.
Drug Development: Assessing the impact of drugs on GABA metabolism and identifying potential therapeutic agents.
Neuroscience Research: Analyzing the role of GABA in regulating neuronal excitability and its function in neural signal transmission.
Nutritional Metabolism Research: Studying the regulatory effects of diet and nutritional supplements on GABA levels.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on GABA and their Metabolites
4. Raw Data
5. Custom Analysis Report
MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into GABA's extensive physiological roles through top-tier analytical techniques and expert services.
How to order?
