δ-D,L-Hydroxylysine Analysis Service

D- and L-δ-Hydroxylysine (D- and L-δ-Hyl) are crucial components of collagen and its metabolism, extensively found in connective tissues. These stereoisomers play significant roles in maintaining the stability and structural integrity of collagen. Changes in D- and L-δ-Hyl levels can be associated with various conditions, such as osteoporosis, arthritis, and skin disorders. Accurate measurement of D- and L-δ-Hyl concentrations is vital for understanding their roles in health and disease, providing a basis for diagnosing and treating related conditions.

 



Figure 1. Molecular Structure of Hydroxylysine

 

MtoZ Biolabs utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to conduct detailed quantitative and qualitative analyses of δ-Hyl. This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of δ-Hyl in complex biological matrices.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity and Precision

HPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of δ-Hyl and differentiating its isomers.

 

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

 

3. Wide Applicability

Suitable for various biological samples, including blood, urine, and tissues.

 

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

 

Sample Submission Requirements

Please ensure that the sample volume is greater than 0.2 g or 0.2 mL.

 

Applications

Clinical Diagnosis: Measure D- and L-δ-Hyl levels in patients with osteoporosis, arthritis, and skin disorders to assist in diagnosis and prognosis.

 

Metabolic Research: Investigate the roles of D- and L-δ-Hyl in collagen metabolism and stability.

 

Drug Development: Evaluate the impact of drugs on D- and L-δ-Hyl metabolism to aid in developing new therapeutic agents.

 

Nutritional Research: Analyze the metabolic changes of D- and L-δ-Hyl under different dietary conditions, providing scientific evidence for nutritional regulation and dietary supplementation.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on D- and L-δ-Hyl

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into δ-D,L-Hydroxylysine's extensive physiological roles through top-tier analytical techniques and expert services.