12-Hydroxyoleic Acid Analysis Service
- Qualitative analysis of the metabolites of 12-hydroxyoleic acid
- Quantitative analysis of 12-hydroxyoleic acid content
- Provide basis for therapeutic applications
- Animal tissue ≥ 50 mg
- Plant tissue ≥ 100 mg
- Serum/ Plasma ≥ 100 μL
- Urine ≥ 1 mL
12-Hydroxyoleic acid is a monohydroxy fatty acid derived from oleic acid, where a hydroxyl group is introduced at the 12th carbon position of the oleic acid chain. This hydroxylation increases the molecule's hydrophilicity while retaining the basic structure and properties of oleic acid, which is an unsaturated omega-9 fatty acid commonly found in various vegetable oils and animal fats. 12-Hydroxyoleic acid plays a crucial role in various biological processes, such as cell membrane composition and signaling pathways, due to its unique structural properties that differ from its parent compound, oleic acid. The introduction of the hydroxyl group can alter the lipid's interaction with other molecules, impacting its role in membrane fluidity and function.
High-performance liquid chromatography (HPLC) can be employed to separate and quantify 12-hydroxyoleic acid in complex biological matrices, enabling detailed analysis of its concentration and distribution in different tissues. Mass spectrometry is used to identify and quantify 12-hydroxyoleic acid in biological samples, providing precise information about its molecular weight and structure. These analytical methods help researchers better understand the physiological and biochemical roles of 12-hydroxyoleic acid in various biological systems.
Figure 1. The Structure of 12-Hydroxyoleic Acid
MtoZ Biolabs offers a leading-edge 12-Hydroxyoleic acid Analysis Service, underpinned by our advanced liquid chromatography-mass spectrometry (LC-MS) platform and expert metabolomics team. Our service provides a comprehensive 12-hydroxyoleic acid analysis, ensuring high sensitivity and accuracy.
Analysis Workflow
Service Advantages
1. Continuously optimized experimental protocols and advanced instrument
2. Professional experimental design
3. Rapid turnaround time
4. High accuracy, specificity, and sensitivity
Applications
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues and other biological samples. It is necessary to select more than 3 materials with the same condition for each sample.
2. Sample Volume
3. Sample Preservation
Store at -80°C to maintain stability.
Note: Provide details on sample collection and handling.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on 12-Hydroxyoleic Acid
4. Raw Data
5. Custom Analysis Report
Through our 12-hydroxyoleic acid analysis service, we aim to facilitate your research and product development processes by providing accurate and reliable results. The 12-hydroxyoleic acid, a hydroxyl derivative of oleic acid, has been studied for its potential anti-inflammatory and anti-cancer effects. This makes our service particularly relevant for researchers in drug discovery and therapeutic development.
How to order?
