20α-Hydroxycholesterol Analysis Service

20α-Hydroxycholesterol (20α-OHC) is a hydroxylated derivative of cholesterol, characterized by the presence of a hydroxyl (–OH) group at the 20th carbon position of the sterol backbone. It belongs to the oxysterol class, which are oxidized derivatives of cholesterol, and is produced through enzymatic processes involving cholesterol hydroxylases, such as cytochrome P450 enzymes, specifically CYP11A1, also known as cholesterol side-chain cleavage enzyme. 20α-OHC is primarily synthesized in steroidogenic tissues (such as the adrenal glands and gonads) and serves as an intermediate in the biosynthesis of steroid hormones.

 

Accurate quantification of 20α-hydroxycholesterol is crucial for diagnosing adrenal diseases that affect steroid hormone production. For example, abnormalities in the production or metabolism of 20α-OHC may indicate defects in the activity of steroidogenic enzymes (such as CYP11A1), leading to conditions like congenital adrenal hyperplasia. Additionally, measuring the levels of 20α-OHC can be used in clinical and research settings to monitor steroidogenic activity. This analysis helps evaluate the effectiveness of treatments targeting steroid production in the adrenal or gonadal glands, such as therapies for adrenal insufficiency or hormone-dependent cancers. The analysis of 20α-hydroxycholesterol is typically performed using mass spectrometry-based techniques, such as liquid chromatography-mass spectrometry (LC-MS) or gas chromatography-mass spectrometry (GC-MS). These methods offer high sensitivity and specificity, enabling the accurate detection of 20α-OHC in biological samples like plasma, serum, or tissue extracts.

 



Figure 1. The Structure of 20α-Hydroxycholesterol

 

MtoZ Biolabs is proud to offer our state-of-the-art 20α-Hydroxycholesterol Analysis Service. Supported by our advanced LC-MS platform and an experienced team of metabolomics specialists, we can assure a comprehensive analysis of 20α-hydroxycholesterol with high sensitivity and accuracy.

 

Analysis Workflow

 



 

Service Advantages

1. Continuously refined protocols and cutting-edge analytical tools  

 

2. Expert experimental planning  

 

3. Rapid processing times  

 

4. Exceptional precision, selectivity, and sensitivity

 

Applications

• Qualitative and quantitative analysis of the metabolites of 20α-hydroxycholesterol.
• Analytical support for drug development and pharmacokinetic studies involving 20α-hydroxycholesterol.
• Provision of essential information for disease research and biomarker discovery related to 20α-hydroxycholesterol metabolism.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is necessary to select more than 3 materials with the same condition for each sample.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Sample Preservation

Ensure to store samples at -80°C to maintain stability.

 

Note: Provide detailed information on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed information on 20α-Hydroxycholesterol

4. Raw Data

5. Custom Analysis Report.

 

Our 20α-hydroxycholesterol analysis service aims to support your research and product development endeavors by providing accurate, reliable, and comprehensive results.