3-Hydroxyisovaleric Acid Analysis Service

3-Hydroxyisovaleric acid (β-hydroxy β-methylbutyric acid, HMB) is a naturally occurring substance in humans, commonly used as a dietary supplement and an ingredient in certain medical foods aimed at promoting wound healing and providing nutritional support for individuals experiencing muscle wasting due to cancer or HIV/AIDS. In healthy adults, HMB supplementation has been demonstrated to increase exercise-induced gains in muscle size, strength, and lean body mass, reduce skeletal muscle damage from exercise, improve aerobic performance, and expedite recovery. Medical reviews and meta-analyses indicate that HMB supplementation also aids in preserving or increasing lean body mass and muscle strength in individuals with age-related muscle loss. Given HMB's significance in muscle growth, immune function, and metabolic health, accurately measuring HMB levels in biological samples is crucial for sports medicine, nutrition, and clinical research.

 

Figure 1. Molecular Structure of 3-Hydroxyisovaleric Acid

 

MtoZ Biolabs offers 3-Hydroxyisovaleric Acid Analysis Service using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). This advanced method combines the separation capabilities of UPLC with the detection power of MS, ensuring high sensitivity and specificity in HMB detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity and Precision

UPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of HMB.

 

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

 

3. Wide Applicability

Suitable for various biological samples, including blood, urine, and tissues.

 

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

 

Sample Submission Requirements

 



For detailed sample submission instructions, please consult our technical team.

 

Applications

1. Sports Medicine: Measure HMB levels to evaluate its effects on enhancing athletic performance and promoting muscle recovery.

 

2. Nutritional Research: Analyze HMB content in food and dietary supplements to guide nutritional intake and supplementation strategies.

 

3. Clinical Research: Monitor changes in HMB levels to aid in diagnosing and treating muscle atrophy, immune dysfunction, and other diseases.

 

4. Metabolic Research: Study the role of HMB in protein synthesis and catabolism to gain a deeper understanding of its biological functions and mechanisms.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 3-Hydroxyisovaleric Acid

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into HMB's extensive physiological roles through top-tier analytical techniques and expert services.