3-Methyl-2-Oxovaleric Acid Analysis Service

3-Methyl-2-oxovaleric acid is an α-keto acid with the molecular formula C₆H₁₀O₃. This compound is characterized by a five-carbon backbone, with a methyl group attached to the third carbon and a keto functional group at the second carbon position. 3-Methyl-2-oxovaleric acid is an important intermediate in the metabolism of branched-chain amino acids (BCAAs), particularly in the catabolism of isoleucine. Isoleucine is transaminated to form 3-methyl-2-oxovaleric acid, a process catalyzed by branched-chain amino acid aminotransferase. Subsequently, 3-methyl-2-oxovaleric acid undergoes further oxidative decarboxylation to form propionyl-CoA and acetyl-CoA, which are involved in energy production within the tricarboxylic acid (TCA) cycle.

 

Elevated levels of 3-methyl-2-oxovaleric acid may indicate metabolic disorders related to branched-chain amino acid metabolism, such as maple syrup urine disease (MSUD). This rare genetic disorder is caused by a deficiency in the branched-chain α-keto acid dehydrogenase complex (BCKDH), which hinders the normal breakdown of branched-chain amino acids, leading to the accumulation of their corresponding α-keto acids, including 3-methyl-2-oxovaleric acid, in the blood and urine. Monitoring the levels of this compound can help diagnose and manage these metabolic disorders. Chromatographic techniques coupled with mass spectrometry, such as gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), are commonly used to detect and quantify 3-methyl-2-oxovaleric acid. These methods offer high sensitivity, selectivity, and accuracy for measuring concentrations in biological samples (such as plasma and urine) for diagnostic and research purposes.

 



Figure 1. The Structure of 3-Methyl-2-Oxovaleric Acid

 

MtoZ Biolabs offers an innovative 3-Methyl-2-Oxovaleric Acid Analysis Service that is supported by our state-of-the-art liquid chromatography-mass spectrometry (LC-MS) platform and an experienced metabolomics team. Our service provides an all-inclusive analysis of 3-methyl-2-oxovaleric acid, ensuring high precision and sensitivity.

 

Analysis Workflow 

 



 

Service Advantages

1. Continuously optimized experimental protocols and advanced instrument

 

2. Professional experimental design

 

3. Rapid turnaround time

 

4. High accuracy, specificity, and sensitivity

 

Applications

• Qualitative and quantitative analysis of 3-methyl-2-oxovaleric acid metabolites
• Analysis of the biological role of 3-methyl-2-oxovaleric acid in different conditions
• Providing crucial data for product development and research

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues and other biological samples. A minimum of 3 samples under identical conditions is required.

 

2. Sample Volume

• Animal tissue ≥ 50 mg
• Plant tissue ≥ 100 mg
• Serum/ Plasma ≥ 100 μL
• Urine ≥ 1 mL

 

3. Sample Preservation

Samples should be stored at -80°C for stability.

 

Note: Details on sample collection and handling must be provided.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information about 3-Methyl-2-Oxovaleric Acid

4. Raw Data

5. Custom Analysis Report

 

With our 3-methyl-2-oxovaleric acid analysis service, we strive to expedite your research and product development by providing comprehensive and high-quality results.