4-Hydroxy-3-Methylbenzoic Acid Analysis Service

4-Hydroxy-3-methylbenzoic acid, also known as m-toluic acid, is an aromatic compound that falls under the category of hydroxybenzoic acids, characterized by a benzene ring substituted with both a hydroxyl group (-OH) and a methyl group (-CH₃) at the meta positions. The molecular formula of 4-hydroxy-3-methylbenzoic acid is C₈H₈O₃, and it has a molecular weight of 152.15 g/mol. This compound is recognized for its structural simplicity and relevance in various biochemical contexts, including its role as an intermediate in the metabolic degradation of toluene by certain bacterial species. In pharmacological and clinical research, 4-hydroxy-3-methylbenzoic acid has drawn attention due to its potential antioxidant properties and its involvement in modulating oxidative stress. The compound may also have applications in anti-inflammatory therapies, given its structural similarity to other hydroxybenzoic acids that are known for their anti-inflammatory effects. Furthermore, recent studies have investigated its potential role in inhibiting bacterial growth, suggesting possible applications in antimicrobial treatments.

 

To analyze and quantify 4-hydroxy-3-methylbenzoic acid in various matrices, sophisticated chromatographic techniques such as high-performance liquid chromatography (HPLC) or gas chromatography (GC) are typically employed. These methods are often coupled with mass spectrometry (MS) to provide high sensitivity and selectivity, enabling accurate detection and quantification even at trace levels. The application of these analytical methods is vital for determining the presence and concentration of 4-hydroxy-3-methylbenzoic acid in environmental and biological samples, elucidating its metabolic pathways, and evaluating its potential therapeutic roles in different biomedical contexts.

 



Figure 1. The Structure of 4-Hydroxy-3-Methylbenzoic Acid

 

Utilizing our advanced liquid chromatography-mass spectrometry (LC-MS) platform, MtoZ Biolabs offers an innovative 4-Hydroxy-3-Methylbenzoic Acid Analysis Service. Guided by our experienced metabolomics team, our service guarantees complete analysis of 4-hydroxy-3-methylbenzoic acid, ensuring high sensitivity and accuracy.

 

Analysis Workflow

 



 

Service Advantages

1. High quality data: We deliver reliable and reproducible results with our advanced LC-MS system.

 

2. Expertise: Our experienced team of scientists ensures the quality and accuracy of our analysis.

 

3. Comprehensive report: We provide a detailed report including the experimental procedure, instrument parameters, and results interpretation.

 

4. Fast turnaround: The process from sample handling to report generation is efficient, shortening the analysis cycle.

 

Applications

• Qualitative and quantitative analysis of 4-hydroxy-3-methylbenzoic acid
• Identification of metabolic pathways of 4-hydroxy-3-methylbenzoic acid
• Pharmacological studies, including drug metabolism and drug-drug interaction studies

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. A minimum of 3 samples is required for each condition.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Sample Preservation

Store at -80°C to ensure stability.

 

Note: Please provide detailed instructions on sample collection and handling.

 

Deliverables

1. Experimental procedures

2. Relevant parameters for liquid chromatography and mass spectrometry

3. Detailed Information on 4-Hydroxy-3-Methylbenzoic Acid

4. Raw data

5. Customized analysis report

 

Through our 4-hydroxy-3-methylbenzoic acid analysis service, we strive to accelerate your research and product development by providing accurate and reliable results.