5-Hydroxy-Tryptamine Analysis Service
- Rapid Analysis Cycle and Cost-Effectiveness
- Stable, Reliable, and Highly Sensitive Results
- Comprehensive One-Stop Service for Extensive Testing Needs
- User-Friendly Operation with Wide Application Scope
- Supported by Extensive Bioanalytical Resources and Advanced Platforms
5-Hydroxy-Tryptamine (5-HT, commonly known as serotonin) is a critical neurotransmitter that plays a significant role in regulating mood, sleep, appetite, and pain perception. 5-HT is synthesized from tryptophan through the enzyme tryptophan hydroxylase (TPH), which converts it to 5-hydroxytryptophan (5-HTP), and then aromatic L-amino acid decarboxylase (AAAD) converts 5-HTP to 5-HT. Variations in 5-HT levels are associated with several disorders, including depression, anxiety, migraines, and irritable bowel syndrome. Accurate measurement of 5-HT concentrations provides insights into its role across different health conditions and offers a scientific foundation for diagnosing and treating related diseases.
Figure 1. Molecular Structure of 5-HT
MtoZ Biolabs utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to conduct detailed quantitative and qualitative analyses of 5-HT and its metabolites. This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of 5-HT in complex biological matrices.
Analysis Workflow
Service Advantages
Sample Submission Requirements
Sample Types
Serum, plasma, urine, tissue homogenates, and other biological samples.
Sample Volume
At least 200 μL of liquid sample or 200 mg of tissue sample.
Sample Preservation
Store and transport samples at -80°C to maintain 5-HT stability.
Applications
Psychiatric Research: Detect changes in 5-HT levels to aid in research and diagnosis of depression, anxiety, and other mental disorders.
Neuroscience Research: Analyze the role of 5-HT in the nervous system and explore its mechanisms in neurotransmitter regulation.
Metabolic Research: Study the role of 5-HT in the tryptophan metabolism pathway and understand its changes under various metabolic conditions.
Drug Development: Evaluate the impact of new drugs on 5-HT metabolism to aid in developing therapies targeting specific neurotransmitter pathways.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on 5-HT and their Metabolites
4. Raw Data
5. Custom Analysis Report
Our 5-Hydroxy-Tryptamine analysis service provides high-quality data support, advancing your scientific research and clinical studies. For any inquiries or requirements, please contact us.
How to order?
