5-methyl-2-thiouridine Analysis Service
- Rapid Analysis Cycle and Cost-Effectiveness
- Stable, Reliable, and Highly Sensitive Results
- Comprehensive One-Stop Service for Extensive Testing Needs
- User-Friendly Operation with Wide Application Scope
- Supported by Extensive Bioanalytical Resources and Advanced Platforms
- Biomarker discovery
- Disease diagnosis and prognosis
- Drug response prediction
- Basic biological research
- Animal tissue ≥ 100 mg
- Plant tissue ≥ 200 mg
- Serum/ Plasma ≥ 200 μL
- Urine ≥ 2 mL
5-Methyl-2-thiouridine (m⁵s²U) is a modified nucleoside found predominantly in the tRNA of certain thermophilic organisms, where it plays a crucial role in enhancing the stability of tRNA structures under extreme conditions. Structurally, m⁵s²U is a derivative of uridine, featuring a methyl group at the 5th position of the uracil ring and a sulfur atom at the 2nd position. These chemical modifications strengthen tRNA’s structural integrity and help maintain accurate codon-anticodon interactions during translation, particularly in high-temperature environments.
Figure 1. The Structure of 5-methyl-2-thiouridine
In addition to its structural role, 5-methyl-2-thiouridine has been implicated in the regulation of gene expression by modulating the efficiency and accuracy of protein synthesis. Studies suggest that such modifications are crucial for the cell’s response to environmental stress and maintaining translational fidelity. The modification of uridine to m⁵s²U in tRNA enhances the molecule's thermal stability and ensures proper function under stressful conditions, making it an important subject of study in molecular biology and biochemistry. Perturbations in its levels are associated with various cellular dysfunctions, indicating its broader role in maintaining cellular homeostasis.
Research into 5-methyl-2-thiouridine provides valuable insights into the biochemical mechanisms that confer stability to nucleic acids, especially in thermophilic organisms. Understanding the role of such modifications in RNA biology can lead to advancements in biotechnology, medicine, and molecular biology. The study of m⁵s²U is particularly relevant for understanding disease mechanisms, protein translation regulation, and potential therapeutic targets.
At MtoZ Biolabs, our cutting-edge mass spectrometry technology coupled with our expertise in metabolomics allows us to provide a precise and comprehensive analysis of m⁵s²U in various sample types. We can accurately quantify m⁵s²U levels, identify its modifications, and provide insights into its metabolic pathway.
Analysis Workflow
Service Advantages
Applications
Our 5-methyl-2-thiouridine analysis service can be applied in a diverse range of research fields, including but not limited to:
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues, and other biological samples. It is necessary to prepare more than 3 biological replicates for each type of sample.
2. Sample Volume
3. Sample Preservation
Store at -80°C to maintain stability.
Note: Please provide details on sample collection and handling.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on 5-methyl-2-thiouridine
4. Raw Data
5. Custom Analysis Report
We look forward to assisting you in your m⁵s²U research endeavors. For more information or to request our services, please contact us at MtoZ Biolabs.
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