7-Ketolithocholic Acid Analysis Service

7-Ketolithocholic acid (7-KLCA) is a secondary bile acid produced by the microbial metabolism of primary bile acids in the intestine. Characterized by a unique 7-keto group, 7-KLCA possesses distinct chemical properties and biological functions compared to other bile acids. Research suggests that 7-KLCA plays crucial roles in the regulation of gut microbiota, cholesterol metabolism, and liver health. Moreover, 7-KLCA has been associated with certain intestinal and liver diseases, and its metabolic changes may offer insights for early diagnosis. Therefore, accurately measuring 7-KLCA levels and metabolic changes is essential for understanding its role in both health and disease.

 

Figure 1. Molecular Structure of 7-Ketolithocholic Acid

 

MtoZ Biolabs offers 7-Ketolithocholic Acid Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in 7-KLCA detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity and Precision

HPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of 7-KLCA.

 

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

 

3. Wide Applicability

Suitable for various biological samples, including blood, urine, feces, and tissues.

 

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

 

Sample Submission Requirements

Make sure the sample volume is greater than 0.2 g or 0.2 mL. For detailed sample submission information, please contact our customer service.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 7-Ketolithocholic Acid

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into 7-KLCA's extensive physiological roles through top-tier analytical techniques and expert services.