a-ketoglutaric Acid (a-KG) Analysis Service
- Highly Efficient Sample Recovery and Processing
- Accurate and Reliable Detection and Quantification
- Capability to Handle Various Sample Types
- Customized Data Analysis Services
- Rapid Service Response and Cost-Effectiveness
- Biomarker discovery
- Disease diagnosis and prognosis
- Drug response prediction
- Basic biological research
- Animal tissue ≥ 100 mg
- Plant tissue ≥ 200 mg
- Serum/ Plasma ≥ 200 μL
- Urine ≥ 2 mL
a-Ketoglutaric acid (a-KG) is a critical metabolite in the tricarboxylic acid (TCA) cycle, also known as the Krebs or citric acid cycle. Structurally, a-KG is a five-carbon dicarboxylic acid and plays a pivotal role in cellular energy metabolism. It acts as an intermediate in the TCA cycle, where it is produced from isocitrate through the action of isocitrate dehydrogenase and subsequently converted to succinyl-CoA by the enzyme a-ketoglutarate dehydrogenase. Beyond its role in energy production, a-KG also participates in amino acid metabolism, oxidative stress response, and cellular signaling pathways.
a-KG is involved in several important biochemical processes, including nitrogen metabolism, where it serves as a key molecule in transamination reactions to synthesize glutamate, a precursor to other amino acids and neurotransmitters. Additionally, a-KG is a critical cofactor for a wide range of enzymes, including dioxygenases, which regulate cellular processes such as DNA repair, epigenetic modifications, and collagen synthesis. Its involvement in such diverse functions makes a-KG a metabolite of great interest in studies of aging, cancer, and metabolic diseases.
The significance of a-KG in cellular metabolism and health has spurred extensive research into its role in regulating metabolic pathways, oxidative stress, and age-related conditions. Recent studies have highlighted a-KG's potential as an anti-aging molecule, its influence on mitochondrial function, and its ability to modulate epigenetic marks. Furthermore, dysregulation of a-KG levels has been implicated in various diseases, including cancer, where mutations in enzymes related to a-KG metabolism can lead to the accumulation of oncometabolites.
Figure 1. The Structure of a-ketoglutaric Acid (a-KG)
At MtoZ Biolabs, we use LC-MS/MS (Liquid Chromatography–Mass Spectrometry) for the quantitative analysis of a-KG due to its high accuracy and specificity. We provide comprehensive a-KG analysis service, including sample preparation, a-KG extraction, LC-MS/MS analysis, and data interpretation.
Analysis Workflow
Service Advantages
Applications
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues, and other biological samples.
2. Sample Volume
3. Storage and Shipping
Samples should be stored at -80℃ and shipped with dry ice.
Note: Please provide details on sample collection and handling.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on a-Ketoglutaric acid
4. Raw Data
5. Custom Analysis Report
At MtoZ Biolabs, we are committed to providing you with the most reliable and accurate a-Ketoglutaric acid (a-KG) Analysis Service. Our expert team will ensure that you receive high-quality data to drive your research forward. Please contact us for more information or to discuss your project needs.
How to order?
