Acetyl-proteomics

Acetyl-proteomics examines the acetylation states of proteins and their roles in biological processes. Protein acetylation, typically occurring on lysine residues and catalyzed by acetyltransferases, regulates gene expression, protein function, and cell signaling. Deacetylation is performed by deacetylases. Studying protein acetylation states reveals dynamic changes in cellular signaling pathways and disease-related molecular mechanisms. Acetyl-proteomics is valuable in cancer, metabolic diseases, neurodegenerative diseases, and other research areas.

Nallamilli, B. R. R. et al. PloS one, 2014.

Figure 1. Multiple Regulatory Functions of Protein Acetylation

MtoZ Biolabs offers Acetyl-proteomics Service using advanced liquid chromatography-tandem mass spectrometry (LC-MS/MS). This technology combines the high separation capability of high-performance liquid chromatography with the high sensitivity of mass spectrometry, enabling efficient and accurate identification and quantification of acetylated proteins.

 Technical Principles

Amino acids modified by acylation can be covalently attached to acyl groups, including acetyl, succinyl, and propionyl, leading to increases in molecular weight by 42.01 Da, 100.01 Da, and 56.03 Da, respectively. Mass spectrometry can precisely detect these mass shifts, allowing for detailed analysis of acylated peptides and their modification sites.

Analysis Workflow

Service Advantages

Sample Submission Requirements

For detailed sample submission instructions, please consult our technical team.

Applications

1. Disease Mechanism Research: Revealing molecular mechanisms of diseases such as cancer, metabolic diseases, and neurodegenerative diseases.

2. Drug Development: Screening potential drug targets and evaluating the impact of drugs on signaling pathways.

3. Cell Signaling Research: Studying dynamic changes in intracellular signaling pathways.

4. Biomarker Discovery: Identifying acetylated protein biomarkers related to disease diagnosis and prognosis.

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Acetylated Proteins

4. Mass Spectrometry Images

5. Raw Data