Affinity Purification Service
Affinity purification is a highly effective method used to isolate specific biomolecules, such as proteins, peptides, antibodies, or other target molecules, based on their unique interactions with a ligand. Affinity purification utilizes the specific binding between a target molecule and an immobilized ligand, such as an antibody, enzyme, or small molecule, which allows for selective purification from complex mixtures. It is a cornerstone of molecular biology, providing high specificity and efficiency in isolating and characterizing biomolecules for further analysis.
In modern research and biotechnology, affinity purification plays a pivotal role in a wide range of applications. Whether in protein identification, enzyme activity studies, or antibody production, affinity purification enables the isolation of the target molecule with high precision. Protein identification is particularly valuable in protein-protein interaction studies, where it allows researchers to isolate not only the protein of interest but also its interacting partners, facilitating the study of complex cellular pathways. Moreover, in drug development and screening, affinity purification is essential for isolating potential drug targets and biomolecular complexes, accelerating the identification of novel therapeutic compounds.
Service at MtoZ Biolabs
MtoZ Biolabs offers customized Affinity Purification Services, leveraging advanced techniques and a wide array of ligands. Our team of experts utilizes a range of affinity ligands, such as antibodies, His-tags, GST-tags, and small molecule ligands, to achieve high specificity in isolating the target molecule. Whether for small-scale research projects or large-scale production, MtoZ Biolabs applies rigorous protocols to ensure the highest level of purity and efficiency. Our flexible approach allows us to handle a variety of sample types, from crude cell lysates to purified proteins, ensuring precise results for every application.
Analysis Workflow
The affinity purification analysis workflow starts with the extraction of total proteins from biological samples, followed by the conjugation of bait-specific antibodies to beads for immunoprecipitation under native conditions. The affinity purification process isolates protein complexes using single or tandem affinity tags, depending on the complexity of the sample. Multiple washing steps are then performed to reduce non-specific interactions and background contaminants. The purified proteins and their interacting partners are analyzed via liquid chromatography-mass spectrometry (LC-MS) for accurate identification and quantification. The resulting data is processed to map protein-protein interaction networks, providing critical insights into cellular processes.
Figure 1. The Analytical Workflow of Protein Complexes by Dimethylated Antibody Affinity Purification and Mass Spectrometry
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Affinity Purification Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. High Specificity: Affinity purification ensures selective isolation of target proteins, minimizing non-specific binding and maximizing the purity of the final product.
3. Reduced Background Contamination: Multiple washing steps and strict control measures effectively reduce background noise, ensuring accurate and reliable results.
4. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all affinity purification analysis data, providing clients with a comprehensive data report.
5. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Applications
1. Analysis of the Protein Interaction Network of Escherichia coli Replication Proteins Under Different Bacterial Growth Conditions Using Affinity Purification-MS
Morcinek-Orłowska, J. et al. Sci Data. 2023.
2. Affinity Purification Enhances Proteomic Identification of Plant RNA Polymerase Pol V Complex and Interacting Proteins
Qin G. et al. Sci Rep. 2017.
FAQ
Q: What types of affinity ligands and tags do you offer for protein purification, and how do you determine the best option for my sample?
We offer a wide range of affinity ligands and tags for protein purification, including common options like His-tags, GST-tags, and FLAG-tags, as well as antibody-based ligands for specific target proteins. The choice of ligand or tag depends on several factors, such as the target protein’s characteristics, the sample type, and the specificity required for the purification. For example, His-tags are ideal for recombinant proteins, while antibody-based affinity purification is used for isolating proteins in complex samples or detecting specific protein interactions. Our team works closely with you to understand your project requirements and determine the best strategy for achieving optimal purity and yield.
How to order?
