Protein Purity and Homogeneity Characterization Service

Protein purity and uniformity are crucial for protein crystallization as well as structural and functional studies. Impure samples can lead to inaccurate results in experiments. Additionally, protein purity and uniformity are two very important characteristics for further protein research and biopharmaceutical applications. The purity of protein samples limits the application of protein products.

 

Common contaminants in protein products include culture media, reagents used in extraction and purification,etc. Beyond impurities, protein non-uniformity may be due to different intracellular reactions and post-translational modifications. It is important to analyze the purity and uniformity of proteins before further processing.

 



Figure 1. Characterization of Protein Purity and Uniformity Marked by pI

 

Services at MtoZ Biolabs

MtoZ Biolabs offers a range of services for characterizing protein purity and uniformity, aiming at detecting contaminants, protein variants, isomers, S-S chain mismatches, truncated proteins, degraded proteins, protein modifications, protein aggregates, and protein precursors.

 

1. Purity and Integrity Analysis Methods

SDS-PAGE, capillary electrophoresis, zinc reverse staining, aldehyde-free silver staining and MALDI-TOF MS.

 

2. Uniformity Analysis

Dynamic light scattering, UV-visible spectroscopy, size exclusion chromatography and static light scattering.

 

Sample Submission Requirements

We can handle samples such as antibodies, antigens, enzymes, growth factors, DNA-binding proteins, blood proteins, and membrane proteins. Common samples include:

1. Proteins From Natural Sources
2. Recombinant Fusion and Tagged Proteins
3. Antibodies of Different Subtypes
4. Membrane Proteins

 

Service Advantages

1. Advanced Technical Platform

We are equipped with various technical platforms to accurately determine the purity and uniformity of proteins.

 

2. Accuracy and Reproducibility

We provide accurate and detailed results on sample purity and uniformity to save time wasted on poor samples.

 

3. Wide Application Scope

Purified and homogeneous proteins can be used for research and medical applications, such as the development and quality control of therapeutic proteins.


MtoZ Biolabs offers various methods to economically and efficiently characterize samples and provides reliable and detailed data reports. For specific service, please contact our technical staff.

• • Peptide Purity Testing Service

  The Peptide Purity Testing Service involves a comprehensive assessment of peptide samples to identify and quantify the main components and impurities. This testing is essential for ensuring peptide quality and consistency, especially in pharmaceutical, research, and diagnostic applications. By performing purity testing, one can determine whether the peptide meets expected quality standards, thus ensuring the reliability of experimental results and minimizing potential interference from impurities. Additiona

• • Peptide Content Analysis Service

  Peptide content analysis service refers to a professional service that tests and evaluates the purity and content of peptide products. Its main purpose is to determine the composition, purity, and whether the peptide samples meet the expected quality standards. This service has important applications in fields such as biomedicine, life science research, and drug development. As drugs or bioactive molecules, the purity of peptides directly affects the reliability of experimental results and the efficacy an

• • Protein Quality Assessment Service

  In the evolving field of life sciences, the integrity and purity of protein samples are paramount for accurate research results and reliable therapeutic applications. MtoZ Biolabs’ Protein Quality Assessment Service is designed to provide researchers and developers with precise, comprehensive evaluations of protein quality. By leveraging advanced mass spectrometry (MS) technologies, we offer a robust assessment framework that delivers detailed insights into protein purity, structural integrity, and...

• • HPLC Protein Analysis Service

  High-performance liquid chromatography (HPLC) is a technique that utilizes chemical and physical interactions between the stationary phase (particles inside the chromatographic column) and the mobile phase (solvent) to achieve high-resolution and high-sensitivity qualitative and quantitative analysis of proteins. It holds great potential in various fields such as bioscience, drug development, and quality control.

• • HPLC Protein Purity Analysis Service

  Protein purity refers to the relative proportion of the target protein in a protein sample, typically expressed as percentage. The higher the purity, the higher the proportion of the target protein in the sample. Measuring protein purity is an important aspect of biochemistry, molecular biology, and biotechnology. By determining protein purity, the quality of the extracted protein sample can be assessed, which can provide a reference for subsequent experimental procedures. High purity protein samples ......