Amino Acid Profiles Analysis Service

Amino acid profile refers to the relative proportions and quantities of different amino acids in proteins or other biological samples, also known as the amino acid composition of proteins. Amino acids are the fundamental building blocks of proteins, and their composition significantly influences the structure, function, and nutritional value of proteins. Quantitative amino acid composition analysis is one of the fundamental aspects of peptide and protein chemical research. By analyzing the amino acid composition, researchers can gain preliminary insights into the chemical structural characteristics of peptides and proteins, as well as the distribution of specific active amino acids, providing valuable scientific information for studying the relationship between protein structure and function. Leveraging high-resolution mass spectrometry and high-performance liquid chromatography (HPLC) technologies, MtoZ Biolabs offers amino acid profiles analysis service to determine the relative proportions and content of various amino acids in biological samples or protein products.

 

Services at MtoZ Biolabs

Based on an advanced analytical platform, MtoZ Biolabs has established a comprehensive amino acid profiles analysis service platform, offering multiple methods for amino acid profile detection, including the following:

 

1. HPLC-Based Amino Acid Profiles Analysis Service

The service utilizes HPLC technology for amino acid profile analysis. One approach involves hydrolyzing protein samples into individual amino acids, followed by derivatization for HPLC detection to obtain qualitative and quantitative results for each amino acid, forming an amino acid profile. The experimental workflow for HPLC-based amino acid profiles analysis service includes hydrolyzing protein samples in 6 N hydrochloric acid under sealed conditions for 22 hours. The hydrolyzed amino acids are derivatized using AccQ Tag reagents, which convert primary and secondary amino acids into stable fluorescent derivatives. Separation is performed using the Waters AccQ•Tag amino acid analysis column, and the quantification of 17 amino acids is achieved using a single-point quantification method.

 



Figure 1. Standard Chromatography of HPLC (Waters AccQ•Tag).

 

2. Amino Acid Profiles Analysis Service Based on Amino Acid Automatic Analyzer

The use of an amino acid automatic analyzer to detect hydrolyzed single amino acids eliminates the need for derivatization with reagents. It directly separates amino acids through liquid chromatography, and the quantification of 17 amino acids is achieved using the single-point quantification method. This approach provides accurate and reliable results while offering a convenient and efficient detection method.

 



Figure 2. Standard Chromatography of Amino Acid Automatic Analyzer

3. Amino Acid Profiles Analysis Service Based on LC-MS Technology

The amino acid profiles analysis service based on liquid chromatography-mass spectrometry (LC-MS) follows a workflow similar to HPLC. Amino acids are derivatized using AccQ Tag reagents. The Waters ACQUITY UPLC I-CLASS system is employed for chromatographic separation, while the Waters XEVO TQ-S Micro tandem quadrupole mass spectrometer performs mass spectrometric analysis. Quantification is achieved using the MassLynx software to calculate peak areas, applying the standard curve method. This approach enables both qualitative and quantitative analysis of more than thirty amino acids and their derivatives with high precision.

 



Figure 3. TIC Diagram of Mass Spectrometric Standards.

 

Service Advantages

1. Advanced Analytical Platform

MtoZ Biolabs has established a cutting-edge amino acid profiles analysis service platform to ensure reliable, fast, and highly accurate analytical services.

 

2. Transparent Pricing

Our pricing structure is transparent, with no hidden or additional fees.

 

3. Customized Research Solutions

MtoZ Biolabs provides tailored services to address your unique research questions and experimental requirements.

 

Sample Submission Suggestions

1. Sample Types and Sample Amount

(1) More than 1 mg of peptide or protein samples.

 

(2) 50 mg of animal tissue, plant tissue and other samples.

 

Applications

1. Validation of Protein Expression Products

Amino acid profiles analysis service can determine the amino acid content and confirm that the proportions of each amino acid align with theoretical values.

 

2. Health and Nutrition Fields

By analyzing the essential amino acid content in various dietary sources (e.g., wheat, rice) under different conditions, amino acid profiles analysis can identify high-quality protein-rich foods.

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!