Other Post-Translational Modification Analysis Service

Post-translational modifications (PTMs) are the various chemical modifications that proteins undergo after translation is complete. Proteins are subject to a range of chemical modifications following synthesis, including phosphorylation, methylation, acetylation, ubiquitination, and among others. These modifications can significantly alter protein structure, function, and interaction, influencing cellular physiology and signal transduction. PTM analysis aims to identify, quantify, and characterize these modifications to better understand protein function and regulation. This process generally includes protein extraction, enzymatic digestion, enrichment of modified peptides, and liquid chromatography coupled with mass spectrometry (LC-MS).

 



Figure 1. The Basic Principles of PTM Analysis of Proteins

 

PTM analysis is crucial for the characterization of biopharmaceuticals, impacting product quality, functionality, safety, and regulatory compliance. It is vital in the development of therapeutic proteins with specific modifications. MtoZ Biolabs utilizes advanced mass spectrometry technology to provide comprehensive PTM profiles capable of identifying and quantifying various modifications such as phosphorylation, acetylation, ubiquitination, methylation, oxidation, deamidation, and N/C-terminal variations (e.g., N-terminal methionine retention and C-terminal lysine loss). Custom analyses, such as those for antibody-drug conjugate (ADC) linkage sites, are also available.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Comprehensive PTM Analysis Results

4. Mass Spectrometry Images

5. Raw Data