Antibody CDR Sequencing Service

The complementarity-determining region (CDR) is the critical part of an antibody that determines its specific binding capability. Each antibody's variable region (VR) contains three CDRs, which together form the antigen-binding site. These CDRs are composed of amino acid sequences found in the variable regions of the antibody's light chain (LC) and heavy chain (HC). Specifically, each chain has three CDRs: CDR1, CDR2, and CDR3. The diversity in the sequences of these CDRs enables antibodies to recognize and bind to a wide range of antigens.

 

In antibody sequencing, accurately determining the sequences of CDRs is essential. This precise identification of CDR sequences provides a deeper understanding of an antibody's specificity and functional properties, which is crucial for the development and optimization of antibody therapeutics. CDR sequencing enables researchers to design antibodies with enhanced affinity and specificity.

 

MtoZ Biolabs has developed an efficient, fast, and accurate antibody CDR sequencing platform based on high-throughput mass spectrometry (MS) technology. Our team of experienced scientists and technical experts has deep expertise in antibody's specificity and functional properties research. We are committed to providing high-quality, efficient services to advance antibody CDR sequencing research and antibody-related drug development. Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.

 

Analysis Workflow

1. Sample Preparation

Purifying the antibody sample to ensure high quality and avoid contamination.

 

2. Enzymatic Digestion

Using specific proteases, such as trypsin, to cleave the antibody into smaller peptides.

 

3. Mass Spectrometry Analysis

Employing high-resolution mass spectrometry (HRMS) to ionize and analyze the peptides.

 

4. Data Interpretation

Utilizing specialized software tools to interpret the mass spectrometry data and reconstruct the antibody's amino acid sequence.

 

5. Validation and Reporting

Conducting further experiments to validate the sequencing results to ensure data reliability.

 

Service Advantages

1. High Coverage

Our antibody CDR sequencing services provide comprehensive coverage, accurately identifying all three CDRs in both the LC and HC of antibodies. This high coverage ensures that every critical region involved in antigen binding is thoroughly analyzed.

 

2. High Throughput

We employ high-throughput MS technology, allowing for the simultaneous analysis of multiple antibody samples.

 

3. Customized Service

MtoZ Biolabs offers a highly customizable antibody CDR sequencing service tailored to meet the specific needs of each client. Our customizable service allows for flexibility in sample preparation, enzymatic digestion, and data analysis protocols. Clients can specify their preferred methods or let our experts recommend the most suitable techniques based on the project’s needs.

 

Applications

1. Antibody Optimization

 



Gopal R, et al. Viruses. 2022.

 

2. Antibody Drug Design

 



Bailly, M. et al. MAbs. 2020.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Antibody CDR Sequencing

4. Mass Spectrometry Images

5. Raw Data