Antibody Epitope Mapping

Antibody epitope mapping is a technique used to identify specific regions (epitopes) on an antigen that bind to antibodies. This mapping provides detailed information about antibody-antigen interactions, including the precise identification of linear or conformational epitopes on the antigen, quantitative data on antibody-epitope binding strength, information on epitope exposure and accessibility, and insights into cross-reactivity with similar epitopes on different antigens. Such information is crucial for understanding immune responses, developing vaccines, and designing therapeutic antibodies.

 

Services at MtoZ Biolabs

Antibody epitope mapping is a critical technology in immunology and biotechnology, offering essential insights for the development of vaccines, therapeutic antibodies, and diagnostic tools. Its precise identification of antibody-antigen interactions is invaluable for both applied and basic research. MtoZ Biolabs provides comprehensive antibody epitope mapping services using techniques such as peptide scanning, phage display, X-ray crystallography, NMR spectroscopy, and mass spectrometry (MS), enabling a thorough elucidation of binding sites from multiple perspectives.

 

Analysis Workflow

1. Preparation of Antigen and Antibody Samples: Isolation and purification of the antigens and antibodies of interest.

2. Selection of Mapping Method: Choosing the appropriate technique based on the nature of the antigen and available resources.

3. Execution of Mapping Procedure: Performing the selected mapping technique to identify the binding regions.

4. Data Analysis: Analyzing the results to determine the specific epitopes recognized by the antibodies.

5. Validation: Confirming the identified epitopes through additional experiments or complementary techniques.

 



Qi, H. et al. Mol Cell Proteomics. 2021.

Figure 1. The Schematic and Workflow of Antibody Binding Epitope Mapping

 

Service Advantages

High Specificity: Precise identification of antibody binding sites.

Detailed Interaction Insights: Understanding the molecular basis of antibody-antigen interactions.

Versatility: Applicable to various antigens and antibodies.

Enhanced Therapeutic Design: Facilitates the development of highly specific and effective therapeutic antibodies.

 

Applications

Rational Vaccine Design: Targeting epitopes that elicit a strong protective immune response.

Optimizing Therapeutic Antibodies: Enhancing efficacy and reducing side effects, ensuring high specificity and affinity.

Improving Diagnostic Accuracy: Developing diagnostic tools that specifically detect relevant epitopes, reducing cross-reactivity.

Elucidating Immune Responses: Providing insights into immune mechanisms and the role of specific epitopes in immune recognition.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Antibody Epitope Mapping

4. Mass Spectrometry Images

5. Raw Data