Application of De Novo Protein Sequencing

De novo protein sequencing does not depend on databases and can directly decode unknown protein sequences.

 

1. Antibody Research

De novo protein sequencing can accurately identify the complementarity-determining regions (CDRs) of antibodies, helping researchers understand antibody specificity and function. Additionally, this technology can analyze antibody variations and optimizations, improving antibody affinity and stability.

 

2. Biomarker Discovery

De novo protein sequencing can identify and characterize novel protein biomarkers, especially when reference databases are lacking. By analyzing protein expression profiles in patient samples, researchers can discover disease-specific biomarkers.

 

3. Post-Translational Modifications (PTMs) Analysis

De novo protein sequencing can identify various PTMs, including phosphorylation, acetylation, and glycosylation. By analyzing these modifications, researchers can gain insights into protein regulatory mechanisms and their roles in diseases.

 

4. Drug Target Analysis

Drug targets are the foundation of drug design and development. De novo protein sequencing can decode the full sequence of drug target proteins, providing detailed molecular information for targeted drug design. By comparing protein differences between healthy and diseased tissues, researchers can discover new drug targets and validate their functions through sequence information.

 

5. Complex Proteomes Research

Complex proteomes, such as those in microbial communities and environmental samples, require de novo sequencing for comprehensive analysis. These samples often lack reference sequence databases, and de novo sequencing can obtain extensive proteome information, helping researchers understand ecosystem functions and microbial interactions.

 

Its significant roles in antibody research, biomarker discovery, PTMs analysis, drug target analysis, and complex proteomes research drive the progress of life sciences. With continuous technological advancements and refinements, the applications of de novo protein sequencing will become more widespread and in-depth. MtoZ Biolabs provides integrate De Novo protein sequencing service.