Application of N-Terminal Sequencing

In the field of proteomics, N-terminal sequencing not only assists scientists in verifying sequence data of proteins but also provides insights into their functions, modifications, and connections to various diseases. N-terminal sequencing is primarily conducted using the Edman degradation process. This method systematically removes and identifies the N-terminal residues of a protein or peptide, with each cycle revealing the amino acid at the chain's beginning. The procedure relies on chemicals like phenylisothiocyanate, which forms a stable, detectable derivative with the amino acid's N-terminal.

 

Applications of N-Terminal Sequencing

1. Protein Identification

Determining a protein’s N-terminal sequence allows researchers to confirm its presence in protein databases or to identify previously unknown proteins.

 

2. Analysis of Post-Translational Modifications

N-terminal sequencing can detect post-translational modifications such as acetylation or phosphorylation, which significantly impact protein functionality.

 

3. Disease Diagnosis and Treatment

Studying the N-terminal variations or abnormal modifications in specific proteins can facilitate the development of novel diagnostic markers and therapeutic targets.

 

It not only deepens our comprehension of protein structure and functionality but also drives forward the development of new therapeutic approaches and strategies for treating diseases. MtoZ Biolabs provides integrate N-Terminal sequencing service.