Application of Peptide Sequencing by Mass Spectrometry

Mass spectrometry (MS) is a powerful analytical tool widely utilized in proteomics research. Through MS sequencing, scientists can precisely determine the peptide sequences of proteins, uncovering their structure and function. This technology plays a significant role in biological research and clinical diagnostics, especially in protein identification, studying protein-protein interactions, biomarker discovery, and drug development.

 

MS sequencing primarily relies on analyzing the mass and charge of peptide fragments using a mass spectrometer. Initially, protein samples are digested into peptide fragments enzymatically, which are then ionized and introduced into the mass spectrometer. The instrument measures each fragment's mass-to-charge ratio (m/z), producing a mass spectrum. Researchers can determine the amino acid sequences of these fragments by comparing the experimental spectra with theoretical spectra in databases.

 

Application Range

1. Protein Identification

MS sequencing is essential for protein identification. By analyzing peptide fragments, scientists can identify and quantify proteins within a sample. This application is fundamental in biological research, such as investigating cell signaling pathways, cell cycle regulation, and other critical biological processes.

 

2. Protein-Protein Interaction Studies

MS sequencing aids in elucidating protein-protein interactions. Analyzing samples from co-immunoprecipitation (Co-IP) or affinity purification (AP) experiments enables scientists to identify components of protein complexes, providing insights into protein networks and cellular functions.

 

3. Biomarker Discovery

MS sequencing is pivotal in the discovery and validation of disease biomarkers. By comparing protein expression profiles between healthy individuals and patients, researchers can identify potential biomarkers. These biomarkers are crucial for early diagnosis, prognosis, and personalized treatment.

 

4. Drug Development

MS sequencing plays a crucial role in drug development. By analyzing drug-protein interactions, researchers can identify potential drug targets and evaluate drug efficacy and safety. Additionally, MS sequencing technology helps monitor drug metabolism, optimizing drug design and therapeutic strategies.

 

MtoZ Biolabs provides integrate peptide sequencing service by mass spectrometry.