Application of Protein Full-Length Sequencing

Full-Length Protein Sequencing (FLPS) is a technique that allows the comprehensive sequencing of a protein molecule from its N-terminus to its C-terminus. Recent advancements in this technology have opened new avenues in biological research, particularly in understanding protein structure-function relationships, elucidating disease mechanisms, and developing new drugs.

 

FLPS is centered on accurately determining the amino acid sequence of proteins. Traditional methods like Edman degradation and mass spectrometry have been fundamental in protein analysis but have limitations. Edman degradation is low throughput, and mass spectrometry can struggle with complex mixtures. Recent innovations, such as single-molecule fluorescence sequencing and nanopore sequencing, have addressed these issues, making efficient and accurate full-length sequencing of complex proteins feasible.

 

Applications of Full-Length Protein Sequencing

1. Protein Structure and Function Research

FLPS is essential for understanding the relationship between protein structure and function. Complete amino acid sequences enable researchers to predict protein tertiary structures and functional domains, providing insights into their roles in cellular processes. For example, sequencing membrane proteins and receptors can reveal their mechanisms in signal transduction and transport.

 

2. Disease Mechanism Elucidation

FLPS is extensively used in disease research, especially for cancers and genetic disorders. Cancer-related protein mutations and abnormal expressions are pivotal in tumor development. FLPS accurately identifies mutation sites and variants, supporting early diagnosis and personalized treatment. Similarly, pathogenic proteins in genetic disorders can be fully analyzed, uncovering their pathological mechanisms.

 

3. Drug Development

Accurate identification and structural analysis of target proteins are vital in drug development. FLPS provides detailed sequence information of target proteins, aiding drug design and optimization. For instance, in antibody drug development, FLPS helps identify precise binding sites and variants, enhancing affinity and specificity.

 

4. Evolutionary Biology Research

FLPS is also significant in evolutionary biology. By comparing homologous protein sequences across species, researchers can understand evolutionary relationships and functional conservation. For instance, sequencing proteins in archaea and fungi can trace functional conservation and variation characteristics, aiding in understanding biological diversity's origin and development.

 

Future Prospects

The continuous advancement of FLPS technology will further propel biomedical research. As the technology matures and its application broadens, more breakthroughs in disease diagnosis, treatment, and biotechnology are anticipated. Combined with other omics technologies like genomics and metabolomics, FLPS will offer a more comprehensive view for systems biology research.

 

FLPS, as a revolutionary technology, has proven its unique value in various biological research fields. By providing comprehensive protein analysis, it offers tools for a deep understanding of life phenomena, driving scientific research forward. MtoZ Biolabs provides integrate protein full-length sequencing service.