Application of Protein Lactylation Analysis

Post-translational modifications (PTMs) of proteins are crucial mechanisms that regulate protein function, localization, and interactions. In recent years, lactylation, an emerging type of protein modification, has garnered significant attention from the scientific community. The discovery of lactylation offers new perspectives on the relationship between metabolism and epigenetic regulation, especially in fields such as oncology, immunology, and metabolic diseases, where lactylation research is rapidly advancing.

 

Protein lactylation refers to the covalent attachment of lactyl groups to lysine residues, thereby regulating the function and stability of proteins. This process primarily occurs in cells with high glycolytic activity, such as tumor cells and immune cells. Lactylation not only impacts protein structure but also alters its interactions with other proteins or DNA, playing a crucial role in various biological processes.

 

Methods for Detecting Protein Lactylation

The detection of protein lactylation currently relies mainly on mass spectrometry (MS) and specific antibody detection. Mass spectrometry provides precise identification and quantification of modification sites, while specific antibodies are useful for large-scale protein screening. With the advancement of technology, methods such as stable isotope labeling by amino acids in cell culture (SILAC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) have been employed, making lactylation detection more sensitive and efficient.

 

Applications of Protein Lactylation Analysis

The applications of protein lactylation analysis are primarily focused on the following areas:

 

1. Applications in Cancer Research

Cancer cells often exhibit abnormal metabolic states, particularly enhanced glycolysis, leading to the accumulation of lactate. This metabolic reprogramming is closely associated with protein lactylation. Studies have shown that the upregulation of lactylation in key proteins within tumor cells may influence cell proliferation, apoptosis, and invasion. By analyzing these lactylation changes, researchers can gain deeper insights into the relationship between tumor metabolism and cell signaling, thereby identifying new targets for cancer diagnosis and treatment.

 

2. Applications in Immune Regulation

In immune cells, lactate functions not only as a metabolic byproduct but also as a signaling molecule that regulates immune responses. Lactylation is considered a critical mechanism of immune metabolic regulation. For example, in macrophages, lactylation may modulate their activation state, thus affecting inflammatory responses and antimicrobial activities. Detailed analysis of lactylation in immune cells could help elucidate the mechanisms underlying immune disorders and guide the development of new immunotherapies.

 

3. Applications in Metabolic Diseases

Metabolic diseases, such as diabetes and obesity, are frequently associated with disrupted metabolic pathways, where aberrant lactylation may play a pivotal role. By detecting and analyzing changes in lactylation in these conditions, we can better understand their pathological processes and discover potential biomarkers and therapeutic targets.

 

Although research on protein lactylation is still in its infancy, with ongoing advancements in detection technologies and expanding application fields, this area of research is poised to provide more substantial evidence for unraveling complex biological mechanisms and fostering the development of novel therapeutic strategies in the future.