Arachidonic Acid Metabolism Service
- State-of-the-art mass spectrometry technology with high sensitivity and accuracy
- Comprehensive data analysis supported by a team of experienced analysts
- Strict quality control to ensure reliable results
- Personalized service to meet various research needs
- Drug discovery and development
- Disease diagnosis and prognosis
- Biomarker identification
- Nutritional and environmental studies
- Type of samples: Plasma, serum, cell, tissue, etc.
- Quantity: At least 200 μl liquid, 1 x 10⁷ cells, or 200 mg tissue.
- Storage and Shipping: Samples should be stored at -80℃ and shipped with dry ice.
Arachidonic acid is a polyunsaturated fatty acid that plays a critical role in cellular signaling and inflammatory responses. It is a key component of the phospholipids that make up cell membranes and serves as a precursor for the synthesis of various bioactive lipid mediators, including prostaglandins, thromboxanes, and leukotrienes. These metabolites are collectively known as eicosanoids, which are involved in a wide range of physiological processes such as immune responses, blood clotting, and regulation of blood pressure.
The initial release of arachidonic acid from membrane phospholipids is catalyzed by phospholipase A₂ (PLA₂). Once freed, arachidonic acid can be oxygenated by three major enzyme systems: cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 monooxygenases (CYP). COX enzymes convert arachidonic acid into prostaglandins and thromboxanes, which are critical for inflammation and hemostasis. LOX enzymes produce hydroperoxyeicosatetraenoic acids (HPETEs) and leukotrienes, which are key mediators in allergic and inflammatory responses. The CYP pathway leads to the formation of epoxyeicosatrienoic acids (EETs), which have diverse roles in cardiovascular and renal functions.
Understanding the intricacies of arachidonic acid metabolism is essential for studying a variety of diseases, including cardiovascular diseases, cancer, and inflammatory disorders. The detailed analysis of this pathway can provide insights into the molecular mechanisms underlying these conditions and help in the development of targeted therapeutic interventions.
Figure 1. The Pathway of Arachidonic Acid Metabolism
Services at MtoZ Biolabs
At MtoZ Biolabs, our Arachidonic Acid Metabolism Service is based on high-performance liquid chromatography-mass spectrometry (HPLC-MS). In this technique, a sample is first separated into its constituent metabolites by liquid chromatography. These metabolites are then ionized and detected by mass spectrometry. The resulting data can be used to identify and quantify the metabolites present in the sample.
Analysis Workflow
Our analysis workflow includes: Sample Collection, Sample Preparation, HPLC-MS Analysis, Data Processing and Analysis, Report Generation
Service Advantages
Applications
Our Arachidonic Acid Metabolism Service can be applied in various fields, including:
Sample Submission Requirements
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Arachidonic Acid Metabolism Analysis
4. Raw Data
5. Custom Analysis Report
How to order?
