Astral DIA: Accurate and Efficient Quantitative Proteomics Solution

The Astral DIA quantitative proteomics service, offered by MtoZ Biolabs, is a cutting-edge solution based on Thermo Fisher Scientific's latest Orbitrap Astral mass spectrometry platform. This service integrates Data Independent Acquisition (DIA) technology with exceptional precision, enabling comprehensive proteome-wide quantitative analysis. By utilizing the highly sensitive Astral instrument, the service is designed to provide extensive and reliable protein quantification for complex biological samples, ensuring accurate and reproducible results for high-throughput proteomics studies.

 

Astral DIA Instrument Advantages

The Astral platform offers several key advantages:

✅High Throughput

Capable of processing 180 samples per day, the system can quantify up to 100,000 proteins in a single run, with a detection capacity of over 8,000 peptides.

 

✅Deep Proteome Coverage 

The instrument achieves over 12,500 peptides per sample, allowing for deep coverage of the proteome, ensuring accurate detection of even low-abundance proteins.

 

✅Superior Sensitivity

With the ability to detect peptides at concentrations as low as 250 femtomoles, the Astral platform provides unparalleled sensitivity for protein quantification.

 

✅Precise Quantification 

The Astral system offers precise quantification capabilities, significantly reducing missing values across datasets and enabling accurate comparison of proteome dynamics.

 

DIA Acquisition Mode Characteristics

The DIA acquisition strategy employed by Astral features:

• Comprehensive Data Collection: DIA technology acquires data across multiple precursor ion ranges, reducing the impact of co-eluting species and enhancing quantitation accuracy.

• Faster Data Acquisition: With full MS scans combined with rapid DIA fragmentation, the Astral system maximizes data points while maintaining coverage and sensitivity.

• Enhanced Data Utilization: The DIA mode eliminates data dependency on traditional DDA methods, allowing for more robust and reproducible quantification, even in highly complex samples.

 



Figure1. Diagram of DIA Acquisition Mode

 

Astral DIA Experimental Workflow

The Astral DIA service is implemented through a streamlined, high-efficiency workflow:

1. Automated Sample Preparation: A fully automated module ensures high-throughput sample processing, enabling standardization and reducing manual handling errors, thus facilitating consistent and reliable results.

 

2. High-Resolution LC-MS/MS Analysis: The Orbitrap Astral mass spectrometer, paired with Neo UHPLC, delivers high-speed and high-resolution MS/MS spectra, maximizing peptide identification and quantification.

 

3. Database Searching: Powerful database search algorithms integrated with advanced software platforms enable high-speed identification and quantification, significantly boosting data analysis efficiency.

 

4. In-depth Data Interpretation: Leveraging MtoZ Biolabs' interactive DIA cloud analysis platform, clients can independently explore deep proteome data, ensuring comprehensive biological insights.

 

MtoZ Biolabs' Astral DIA service sets a new standard in quantitative proteomics, offering unmatched sensitivity, throughput, and precision, making it the ideal solution for large-scale, high-depth proteomics research. This service is suitable for a wide range of applications, from biomarker discovery to functional proteomics and drug development, ensuring researchers can gain the most detailed understanding of the proteome dynamics in their samples.