BioID&TurboID
BioID&TurboID are biochemical techniques widely used to investigate intracellular protein interaction networks. These methods enable researchers to map complex protein interaction networks by labeling proteins that are in close proximity to or interact with specific target proteins. BioID, short for Biotin Identification, utilizes a mutant form of biotin ligase (BirA*) to attach biotin tags to nearby proteins. These tagged proteins can then be isolated and identified through affinity capture techniques. TurboID, an optimized version of BioID, offers enhanced performance over the original method.
In scientific research, both BioID&TurboID have broad applications, particularly in proteomics and cell biology. These techniques are crucial for studying intracellular signaling pathways, uncovering protein interactions related to diseases, and assisting in the development of new therapeutic targets. For example, in research on neurodegenerative diseases, cancer, and immune system disorders, BioID&TurboID have facilitated the identification of key proteins and their associated interaction networks. Furthermore, these techniques show considerable potential in biotechnology and pharmaceuticals. By directly labeling and capturing interacting proteins in live cells, they provide valuable tools for drug target identification and the study of drug mechanisms. In drug development, BioID&TurboID can aid in identifying potential drug targets, examining interactions between drugs and target proteins, and assessing the efficacy and specificity of drugs.
BioID&TurboID each have distinct advantages. One of BioID’s main benefits is its high specificity and versatility, as it does not require complex procedures such as cell lysis or protein purification for labeling. This makes BioID particularly useful for studying protein interaction networks under steady-state conditions. TurboID, on the other hand, offers a significant advantage in its labeling speed. The biotinylation reaction in TurboID occurs much faster than in BioID, completing the labeling process within just 10 minutes. This rapid labeling capability is ideal for studying protein interactions that change quickly over time, providing higher temporal resolution and allowing the detection of transient interactions that might be missed by traditional methods. Moreover, TurboID retains high labeling efficiency even at low temperatures and biotin concentrations, minimizing potential interference with the cell's physiological state.
When performing BioID&TurboID experiments, researchers must carefully consider several experimental factors to ensure the accuracy and reproducibility of results. The expression level of the target protein is critical, as extreme levels-either too high or too low-can lead to non-physiological interactions or undetectable ones. Researchers should select appropriate vectors and promoters to regulate target protein expression levels effectively. Non-specific labeling is another common challenge in these experiments. To address this, researchers should include appropriate controls, such as empty vector controls and controls with known non-interacting proteins, to validate the specificity of the labeling.
MtoZ Biolabs offers extensive experience and expertise in these techniques. We are committed to providing high-quality data and comprehensive analysis to help clients achieve groundbreaking research results. By partnering with us, researchers gain personalized technical support and tailored solutions, ensuring the success of each project. MtoZ Biolabs looks forward to collaborating with you to explore the frontiers of life sciences.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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