Biopharmaceutical N/C-Terminal Sequencing Service

The N-terminal of a protein molecule typically encompasses the translation initiation and targeting signals, playing a crucial role in the protein's synthesis, processing, localization, and functional integrity. Conversely, the C-terminal, often embedded with functional domains and signaling motifs, significantly influences the protein's stability, functionality, and involvement in cellular signaling pathways. N-terminal signal peptides direct proteins to specific intracellular locales, such as the endoplasmic reticulum or mitochondria. Proteolytic cleavage of these peptides is a vital step in the maturation of many proteins. Protein activity can also depend on specific N-terminal structural domains or modifications like acetylation. Similarly, the C-terminal may feature signaling sequences that govern protein interactions, impacting protein stability and degradation.

 

For sequencing, traditional methods like Edman degradation and various forms of mass spectrometry have been used. However, due to their limitations, liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has become the preferred approach for N/C-terminal sequencing. This technique allows for the detailed analysis of N-terminal peptide fragments, offering high sensitivity and accuracy, suitable for complex sample matrices.

 

As a outstanding provider of protein sequencing services, MtoZ Biolabs leverages its state-of-the-art experimental facilities and sophisticated techniques to deliver top-quality, comprehensive biopharmaceutical N/C-terminal sequencing services.

 



Vecchi, MM. et al. Anal Chem. 2019.

Figure 1. The Workflow for Sequencing of N-terminal by Mass Spectrometry

 

Analysis Workflow

1. Sample Preparation: Depending on the characteristics of the protein and the specific needs of the customer, various sample processing methods are used, such as reduction, alkylation, and enzymatic digestion.

2. Sequence Enrichment: Employing specialized enrichment technologies, such as immobilized enzyme reaction containers (IMERs), to accurately capture the target protein's N-terminal or C-terminal peptide segments.

3. Liquid Chromatography-Mass Spectrometry Analysis: Utilizing high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS/MS) for detailed analysis to ensure precise sequence identification.

4. Data Processing and Analysis: Advanced bioinformatics tools are applied to conduct in-depth analysis of the obtained mass spectrometry data, accurately determining the protein's N-terminal and C-terminal sequences.

 

Service Advantages

1. High Precision Identification: We utilize the most advanced mass spectrometry technology to ensure high accuracy and repeatability of analysis results.

2. Customized Services: We offer personalized sample processing and analysis plans based on the different characteristics and research needs of proteins.

3. Fast and Efficient: Our optimized experimental procedures and a professional technical team ensure rapid sample processing and data analysis, shortening the R&D cycle.

4. Professional Data Interpretation: We provide not only raw data but also comprehensive analysis reports and professional interpretation of results, aiding customers in deeply understanding their research findings.

 

Applications

1. Drug Development: Providing precise protein structural analysis support for the development of new drugs, especially important in the development of biosimilars and antibody drugs.

2. Quality Control: Ensuring that the quality of biopharmaceutical products during production meets strict drug safety standards.

3. Research and Development: Helping researchers in protein engineering, drug design, and the study of disease mechanisms, accurately understanding the structural and functional relationships of proteins.

 

Through MtoZ Biolabs' biopharmaceutical N/C-terminal sequencing service, we promise to provide reliable, efficient, and comprehensive analytical support to facilitate research and development in the biopharmaceutical industry.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Biopharmaceutical N/C-Terminal Sequencing

4. Mass Spectrometry Images

5. Raw Data