Metabolic Bone Disease Analysis Service

Metabolic bone disease encompasses a range of bone disorders caused by congenital or acquired factors that disrupt normal bone metabolism and biochemical states, leading to disorders in bone biochemistry and metabolism and the occurrence of bone disorders. These disorders stem from abnormalities in three key areas: bone resorption, bone growth, and mineral deposition. Increasingly, research has identified numerous cytokines involved in the processes of bone remodeling and metabolism. Cytokines such as IL-1α, IL-1β, TNF-α, and TNF-β significantly stimulate bone resorption and inhibit bone formation. IL-6, synthesized by osteoblasts (OBs), is promoted by parathyroid hormone (PTH), which encourages OBs to secrete IL-6, thereby facilitating the differentiation and proliferation of osteoclasts (OCs). The role of colony-stimulating factors (CSFs) is critical in the early phases of OC formation and differentiation.

 

MtoZ Biolabs utilizes flow cytometry to simultaneously quantify cytokines and proteins related to metabolic bone disease, providing higher sensitivity and a broader detection range. The following table lists the cytokines detectable in human samples, and MtoZ Biolabs also offers testing for other species. Free project evaluation, welcome to learn more details!

 

Metabolic Bone Disease - Cytokine Detection Parameters via Flow Cytometry

 



Figure 1. Multifactor Flow Cytometry Kit for Human Samples

 



Figure 2. Single Factor Flow Cytometry Kit for Human Samples


 

Sample Submission Requirements

1. Only 25 µL of sample required, while traditional ELISA requires 50 µL or 100 µL samples.

2. Suitable for serum, plasma, cell culture supernatant, and various body fluids.

3. Body fluid samples are stored at -80°C and transported using dry ice. 

 

Deliverables

1. Experimental Procedures

2. Raw Data (Fluorescence Luminescence Spectra)

3. Technical Report

4. Result of Absolute Content of Each Detection Indicator (Excel Spreadsheet)