Brassinolide Analysis Service
- Absolute quantification of brassinolide
- Differential metabolites screening
- Optimal analyses such as KEGG pathway analysis and hierarchical clustering
Brassinolide is the most biologically active brassinosteroid, initially discovered in the pollen of Brassica napus (rapeseed). It has a complex chemical structure based on a steroid backbone with multiple hydroxyl groups, which are crucial for its biological activity. Brassinolide is involved in various physiological processes in plants, including regulating cell elongation and division, promoting photomorphogenesis, enhancing stress resistance, promoting flower and fruit development, and controlling senescence. Brassinolide functions through a specific signaling pathway that involves the BR receptor BRI1 (Brassinosteroid Insensitive 1) located on the plasma membrane. When brassinolide binds to BRI1, it triggers a cascade of signal transduction, ultimately activating transcription factors that regulate gene expression, affecting plant growth and development.
Figure 1. The Structure of Brassinolide
MtoZ Biolabs offers a premier Brassinolide Analysis Service, bolstered by our advanced ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) platform and our expert metabolomics team. Our service provides a comprehensive brassinolide analysis, ensuring high sensitivity and accuracy.
Analysis Workflow
Figure 2. Workflow for Brassinolide by UPLC-MS/MS Analysis
Service Advantages
1. Expert Team: Our team scientists have deep understanding and extensive experience in MS and metabolomics.
2. Advanced Techniques: We employ state-of-the-art UPLC-MS/MS platforms that ensure high sensitivity and specificity of detection.
3. Customizable Service: Our service is flexible to accommodate specific research needs and goals.
4. Fast Turnaround Time: We strive to deliver results in a timely manner without compromising on the quality.
5. Data Confidentiality: We strictly adhere to data security and confidentiality protocols.
Applications
Sample Submission Requirements
1.Fresh plant tissues >2 g. Samples should be frozen in liquid nitrogen immediately after collection, and then transferred to -80°C for storage.
2. Plant seeds >2 g.
At least 3 biological replicates.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Brassinolide
4. Raw Data
5. Custom Analysis Report
Through our brassinolide analysis service, we aim to accelerate your research and product development processes by providing accurate and reliable results.
How to order?
