C-Terminal Sequencing

C-terminal sequencing is a specialized technique used to determine the C-terminal amino acid sequence of proteins or peptides. This technique is crucial for confirming the integrity and functionality of proteins, particularly in the fields of biopharmaceuticals and structural biology. C-terminal sequencing can reveal information about a protein's stability, activity, and interactions with other biomolecules, which are vital for the development of new drugs and quality control of biologics. At MtoZ Biolabs, we utilize cutting-edge mass spectrometry technology to provide precise C-terminal sequence analysis, helping scientists make breakthroughs in protein research and drug development.

 

Services at MtoZ Biolabs

As a leader in the field of bioanalysis, MtoZ Biolabs is committed to delivering high-precision protein analysis services to help researchers and pharmaceutical companies worldwide solve complex biological challenges. Our C-terminal sequencing service reflects our core strengths: the use of advanced technology, rigorous operational procedures, and a deep understanding of our clients' research needs. Choosing MtoZ Biolabs, you will receive comprehensive professional support from sample preparation to data analysis. Our team of technical experts will ensure that your research results are both accurate and reliable. Whether it's for protein structural studies, new drug development, or quality control of biologics, MtoZ Biolabs is your ideal partner.

 

Analysis Workflow

Our C-Terminal Sequencing service follows a streamlined and efficient process:

 

1. Sample Submission: Researchers submit their protein samples, along with the necessary information.

2. Sample Preparation: Our lab team prepares the samples for analysis, including protein purification and tryptic digestion.

3. Sequencing Analysis: The samples are then subjected to the ETD or HCD mass spectrometry process for C-terminal sequencing.

4. Data Analysis: Our bioinformatics experts carry out in-depth data analysis, providing comprehensive protein sequence information.

5. Report Generation: A detailed report, including the methods, results, and conclusions, is prepared and shared with the clients.

 



Figure1. Protein C-Terminal Sequencing Workflow

 

Application Scenarios

C-Terminal Sequencing is crucial in various research fields, including protein engineering, antibody production, vaccine development, and drug discovery. In addition, it aids in understanding protein function, localization, interaction, and stability.

 

Sample Submission Requirements

For our C-Terminal Sequencing service, clients are required to provide a protein sample with a minimum concentration of 1 mg/mL and a minimum volume of 50 μL. The sample should be devoid of any contaminating proteins or substances that might interfere with the mass spectrometry analysis.

 

By selecting MtoZ Biolabs for C-terminal sequencing services, you will benefit from internationally leading technology support and professional services, ensuring that your research and product development receive the most accurate C-terminal protein sequence data.