Castasterone Analysis Service

Castasterone is an important plant steroid hormone belonging to the brassinosteroids (BRs) family, regulating plant growth, development, and various physiological processes such as cell division, cell expansion, stem elongation, leaf expansion, and reproductive development. The chemical structure of castasterone features a typical steroid backbone with multiple hydroxyl groups, which gives it significant biological activity in plants. In plants, castasterone is produced through the biosynthesis pathway of brassinosteroids and is eventually converted into more biologically active brassinosteroids, such as Brassinolide. Castasterone itself plays a crucial role in promoting cell division and expansion, being essential for overall plant growth and development. The content and metabolic rate of castasterone can significantly influence plant growth speed, stem thickness, and response to environmental conditions. Research on castasterone and its biosynthetic pathway is of great importance in agriculture and horticulture. By regulating the synthesis of castasterone, it is possible to effectively promote crop growth, increase yields, and enhance stress resistance. Additionally, studying the metabolic pathway of castasterone provides a theoretical basis for developing new plant growth regulators.

 



Figure 1. The Structure of Castasterone

 

MtoZ Biolabs provides a top-tier Castasterone Analysis Service, leveraged by our state-of-the-art ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) platform and the expertise of our metabolomics team. Our service provides an in-depth castasterone analysis, ensuring high sensitivity and precision.

 

Analysis Workflow 

 



Figure 2. Workflow for Castasterone by UPLC-MS/MS Analysis

 

Service Advantages

1. Expert Team: Our team scientists have deep understanding and extensive experience in MS and metabolomics.

 

2. Advanced Techniques: We employ state-of-the-art UPLC-MS/MS platforms that ensure high sensitivity and specificity of detection.

 

3. Customizable Service: Our service is flexible to accommodate specific research needs and goals.

 

4. Fast Turnaround Time: We strive to deliver results in a timely manner without compromising on the quality.

 

5. Data Confidentiality: We strictly adhere to data security and confidentiality protocols.

 

Applications

• Absolute quantification of castasterone
• Differential metabolites screening
• Optimal analyses such as KEGG pathway analysis and hierarchical clustering

 

 Sample Submission Requirements

1. Fresh plant tissues >2 g. Samples should be frozen in liquid nitrogen immediately after collection, and then transferred to -80°C for storage.

 

2. Plant seeds >2 g.

 

At least 3 biological replicates are required.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Castasterone

4. Raw Data

5. Custom Analysis Report

 

Our castasterone analysis service aims to support your research and product development processes by providing accurate and reliable results.