Catecholamine Analysis Service

Catecholamines, including adrenaline, noradrenaline, and dopamine, are crucial physiological compounds that act as significant neurotransmitters in the brain and nervous system. These compounds also play essential roles in the cardiovascular system, metabolic regulation, and stress response. Abnormal levels of catecholamines are associated with diseases like Parkinson's disease, depression, and hypertension. Therefore, precise measurement of catecholamines in biological samples is vital for disease diagnosis, drug development, and physiological research.

 

 

Figure 1. Several Major Catecholamine Structures

 

MtoZ Biolabs employs advanced techniques for catecholamine detection through isotope-coded derivatization-dispersive liquid-liquid microextraction (DLLME) combined with ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The use of d0/d3-10-methylacridone-2-sulfonyl chloride (D0/d3-MASC) as the derivatization reagent enhances the sensitivity and specificity of catecholamine detection.

 

Analysis Workflow

1. Sample Preparation

Preparation of samples and internal standards with d0-MASC and d3-MASC.

 

2. Dispersive Liquid-Liquid Microextraction

Enrichment and purification of samples using ultrasound-assisted technology.

 

3. UPLC-MS/MS Detection

Optimized conditions for chromatography and mass spectrometry ensure rapid separation and high-sensitivity detection.

 

4. Data Processing

Construction of standard curves, data analysis, and result reporting.

 



 

Service Advantages



 

Sample Submission Requirements



 

For detailed sample submission instructions, please consult our technical team.

 

Applications

Neurodegenerative Disease Research: Analyze neurotransmitter changes to study the mechanisms of diseases like Parkinson's and depression.

 

Drug Development and Evaluation: Assess the impact of new drugs on catecholamine levels, optimizing dosage and mechanisms of action.

 

Biomarker Discovery: Identify disease-related biomarkers to assist in diagnosis and prognosis.

 

Stress Response Research: Study catecholamine changes under stress to develop intervention strategies.

 

Precision Medicine: Customize drug interventions based on individual catecholamine profiles to enhance personalized treatment outcomes.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Catecholamine

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to providing high-quality catecholamine analysis services. With cutting-edge technology, a skilled technical team, and comprehensive service processes, we meet your research and clinical needs. We look forward to collaborating with you to advance life science research.