Cis-Aconitic Acid Analysis Service

Cis-Aconitic acid is a vital organic acid and a key intermediate in the tricarboxylic acid (TCA) cycle. It is formed from citric acid through the action of aconitase and is subsequently converted into isocitric acid. This compound plays a crucial role in energy metabolism, cellular respiration, and metabolic regulation. Abnormal levels of cis-aconitic acid are linked to various metabolic diseases, mitochondrial dysfunction, and apoptosis. Thus, accurate detection and quantification of cis-aconitic acid are essential for understanding cellular metabolic states, diagnosing diseases, and assessing health.

 

Changes in cis-aconitic acid levels can indicate alterations in the TCA cycle, such as energy metabolism disorders and oxidative stress. Research has shown that the accumulation of cis-aconitic acid may be associated with genetic and metabolic diseases, including mitochondrial disorders and diabetes. Early diagnosis and monitoring of these conditions are vital for effective patient management and treatment. Additionally, cis-aconitic acid is significant in drug metabolism research and environmental science, such as evaluating the impact of drugs on metabolic pathways and assessing pollution levels in environmental samples. Therefore, precise measurement and quantification of cis-aconitic acid are invaluable for clinical research, environmental monitoring, and drug development.

 

Figure 1. Molecular Structure of Cis-Aconitic Acid

 

MtoZ Biolabs offers Cis-Aconitic Acid Analysis Service using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). This advanced method combines the separation capabilities of UPLC with the detection power of MS, ensuring high sensitivity and specificity in cis-aconitic acid detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity and Precision

UPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of cis-aconitic acid.

 

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

 

3. Wide Applicability

Suitable for various biological samples, including blood, urine, and tissues.

 

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissue homogenates, and other biological samples.

 

2. Sample Volume

At least 200 μL of liquid sample or 200 mg of solid sample.

 

3. Sample Preservation

Store at -80°C to maintain stability.

 

Note: Provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Cis-Aconitic Acid

4. Raw Data

5. Custom Analysis Report

 

Our Cis-Aconitic Acid Analysis Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.