Comparison of Mass Spectrometric MRM with Other Quantitative Methods in Proteomics

Mass Spectrometry MRM quantification is an accurate and repeatable quantification technique in proteomics. With its high sensitivity, high diversity, and high throughput advantages, MRM is widely accepted by scientific research institutions worldwide.

 

Service Advantages

1. High Sensitivity

MRM can detect low-abundance protein markers amidst background noise and is hailed as the “cell counter in mass spectrometry.”

 

2. High Diversity

Without the need for pre-labeling, MRM can quantify a variety of proteins, offering a vast research space.

 

3. High Throughput

MRM can analyze hundreds of target proteins simultaneously, greatly improving research efficiency.

 

Comparison with Other Proteomics Quantification Methods

1. Labeling Quantification

As the name suggests, this method requires pre-labeling of target proteins. Although MRM, which does not require pre-labeling, has more flexibility, labeling quantification has incomparable advantages for pre-labeled protein fluorescence quantification.

 

2. Immunological Quantification

Immunological quantification represented by ELISA has its distinct advantages and disadvantages. They can accurately quantify specific proteins, but each protein requires the development and validation of new immunoassays, which is relatively labor-intensive.