Comprehensive High-Resolution Broad-Spectrum Lipidomics Absolute Quantitative Analysis Service by MtoZ Biolabs
- Q-Exactive Plus Mass Spectrometer (Thermo Scientific) for high-resolution mass spectrometry analysis.
- UHPLC Nexera LC-30A (SHIMADZU) for ultra-high-performance liquid chromatography.
- Eppendorf 5430R for low-speed centrifugation in sample preparation.
- Waters ACQUITY UPLC CSH C18 Column for chromatographic separation of lipid species, providing optimal resolution for complex lipid mixtures.
Lipidomics, a burgeoning field within systems biology, is crucial for understanding the complex interactions of lipids in biological systems. Lipids are hydrophobic or amphiphilic molecules that play key roles in cellular processes, including energy storage, membrane structure, and signaling. The accurate profiling of lipids is imperative for deciphering their biological roles in health and disease, particularly in inflammation, neurological disorders, metabolic dysfunction, and cancer.
MtoZ Biolabs offers a high-resolution broad-spectrum lipidomics absolute quantitative analysis service. This cutting-edge platform combines non-targeted and targeted lipidomics approaches using advanced mass spectrometry techniques to provide accurate, reproducible, and comprehensive lipid profiles. Our service is built on years of expertise in mass spectrometry and is designed to support researchers in obtaining high-quality lipidomics data for biomedical, pharmaceutical, and clinical studies.
1. Experimental Principles
Lipids, comprising a wide array of classes such as fatty acids, glycerides, phospholipids, sphingolipids, and sterols, are structurally and functionally diverse. According to the LIPID MAPS classification, eight major lipid categories include fatty acyls (e.g., oleic acid), glycerolipids (e.g., triacylglycerol), glycerophospholipids (e.g., phosphatidylcholine), sphingolipids (e.g., ceramide), sterol lipids (e.g., cholesterol), and more.
In the context of disease pathophysiology, lipid homeostasis is crucial for maintaining cellular function, with dysregulation contributing to conditions such as cardiovascular diseases, neurodegeneration, metabolic syndrome, and cancer. Lipidomics, the systematic study of lipid species, allows researchers to map lipid metabolism and explore lipid-protein and lipid-lipid interactions that influence disease progression.
Our analytical strategy utilizes both untargeted and targeted lipidomics. Untargeted lipidomics allows for the detection of hundreds of lipid species without pre-selection, offering a broad view of lipid metabolic changes across diverse biological samples. In contrast, targeted lipidomics focuses on the precise quantification of specific lipids of interest, delivering high specificity and sensitivity.
Key technologies in our lipidomics platform include ultra-performance liquid chromatography (UPLC) coupled with high-resolution tandem mass spectrometry (LC-MS/MS), powered by advanced software tools like LipidSearch for peak identification, alignment, and quantification of lipid species. With these technologies, we are capable of resolving lipid molecular species based on their mass-to-charge (m/z) ratios, allowing for both relative and absolute quantification.
2. Analysis Workflow
The high-resolution broad-spectrum lipidomics absolute quantitative analysis workflow at MtoZ Biolabs includes the following steps:
（1）Sample Preparation
Samples are prepared according to specific lipid extraction protocols to ensure the integrity of the lipid content.
（2）Quality Control (QC) Setup
QC samples are prepared and used throughout the analysis to monitor instrument performance and ensure consistency across runs.
（3）Lipid Extraction and Separation
Lipids are extracted from biological matrices using organic solvents, followed by chromatographic separation using the UPLC system.
（4）LC-MS/MS Analysis
Lipid species are analyzed using Thermo Scientific Q-Exactive Plus mass spectrometer in conjunction with UPLC for high-resolution separation and detection.
（5）Data Processing and Quantification
Data analysis is performed using LipidSearch software, which allows accurate identification and quantification of lipid species through peak alignment and mass spectral matching.
The use of internal standards and isotope-labeled lipids ensures accurate quantitation of both relative and absolute lipid concentrations, with each species quantified based on chromatographic peak area and concentration calibration curves.
3. Instruments and Reagents
Our high-resolution lipidomics service employs state-of-the-art instruments to ensure precise and reproducible results:
Reagents used include high-purity solvents such as ethanol, isopropanol, and methanol from Thermo Fisher to ensure contamination-free sample processing.
4. Data Analysis and Quantification
Data analysis is conducted using LipidSearch (Thermo Fisher Scientific), which is optimized for processing high-resolution mass spectrometry data. This software enables the identification of over 14 lipid subclasses, ensuring robust annotation of lipid species. It also supports both automated and manual curation of lipid features, allowing precise peak alignment and lipid quantification.
Our method supports a broad range of lipid species detection, including approximately 70,000 unique lipid molecular species, covering glycerophospholipids, glycerolipids, sphingolipids, sterol lipids, and others. Additionally, the targeted quantification approach allows for the absolute quantification of specific lipids, down to nanomolar concentrations, providing high sensitivity and accuracy in biomarker validation and mechanistic studies.
5. Expanded Research Recommendations
MtoZ Biolabs' lipidomics service is uniquely positioned to support advanced research in a variety of biomedical fields. By integrating lipidomics with other omics approaches such as proteomics and transcriptomics, researchers can gain a comprehensive understanding of lipid function in biological systems. Building upon the comprehensive analysis of lipids, there are several future research directions and expanded applications that can benefit from MtoZ Biolabs' high-resolution lipidomics service. These include but are not limited to:
✅ Multi-omics Integration: Exploring Disease Mechanisms and Models
Lipidomics can be integrated with other omics approaches, such as proteomics and transcriptomics, to create a multidimensional view of biological processes. This integrative approach enables a deeper understanding of lipid-protein and lipid-RNA interactions, providing insights into the regulatory networks of diseases. By investigating these relationships, researchers can better elucidate the mechanisms driving disease onset and progression. For example, integrating lipidomics data with transcriptomic profiles of immune cells could offer new perspectives on immune-lipid interactions in inflammation.
✅ Lipid Remodeling in Inflammatory Responses
Lipid metabolism is central to the regulation of inflammation. Studying lipid remodeling during inflammatory responses, such as the activation of specific lipid species that mediate or suppress inflammation, can provide new targets for therapeutic interventions. With the advancement of LC-MS/MS technologies, lipidomics allows for the detailed characterization of lipid-mediated signaling pathways involved in the immune response, enabling researchers to explore novel biomarkers and potential therapeutic targets for diseases such as arthritis, sepsis, and chronic inflammation.
✅ Intermediate Chain Fatty Acids: A Focus on Metabolic Regulation
Under specific physiological conditions, LC-MS/MS-based lipidomics enables the precise detection of intermediate chain fatty acids and their metabolic derivatives. These lipids play critical roles in energy homeostasis and signaling. Research focusing on how these lipid intermediates fluctuate in response to metabolic stress, dietary changes, or pathological conditions could reveal important regulatory pathways in metabolic diseases, such as diabetes and obesity.
✅ Lipid Dysregulation in Neurodegenerative Diseases
Sphingolipids, glycerophospholipids, and other lipid classes are closely linked to the pathology of neurodegenerative diseases, including Alzheimer's and Parkinson's disease. By applying lipidomics to brain tissue or cerebrospinal fluid samples, researchers can uncover dysregulated lipid pathways that contribute to neuronal degeneration. High-resolution lipidomics can help pinpoint key lipid alterations, advancing our understanding of disease mechanisms and paving the way for the development of lipid-based biomarkers for early diagnosis and treatment monitoring.
Furthermore, the combination of MRM (Multiple Reaction Monitoring) and PRM (Parallel Reaction Monitoring) in our platform ensures the highest precision for lipid quantification in targeted studies. These methods provide comprehensive insights into lipid metabolism and are pivotal for discovering disease biomarkers and therapeutic targets.
MtoZ Biolabs' high-resolution broad-spectrum lipidomics absolute quantitative analysis service provides researchers with a powerful tool for deciphering lipid biology and its role in health and disease. By employing state-of-the-art mass spectrometry and chromatography techniques, along with advanced data analysis software, our service ensures accurate, reproducible, and in-depth lipid profiling. This service is indispensable for researchers seeking to unlock the complexities of lipid metabolism and translate lipidomics discoveries into clinical applications.
Contact MtoZ Biolabs today to learn more about how our lipidomics platform can support your research endeavors.
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