Cortisone Analysis Service

Cortisone (17-hydroxy-11-dehydrocorticosterone) is a glucocorticoid hormone secreted by the adrenal cortex, crucial for regulating immune responses, inflammatory processes, and the metabolism of carbohydrates, proteins, and fats. As an inactive precursor, cortisone is converted into its active form, cortisol, by the enzyme 11β-hydroxysteroid dehydrogenase within the body, where it then exerts physiological effects on target tissues. Clinically, cortisone level measurement is often employed to assess adrenal function, aid in the diagnosis of endocrine disorders such as Addison's disease and Cushing's syndrome, and monitor the effectiveness of glucocorticoid therapy.

 

Figure 1. Molecular Structure of Cortisone

 

MtoZ Biolabs offers Cortisone Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in cortisone detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity and Precision

HPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of cortisone.

 

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

 

3. Wide Applicability

Suitable for various biological samples, including blood, urine, and tissues.

 

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissue extracts, and other biological samples.

 

2. Sample Volume

At least 200 μL of liquid sample or 200 mg of solid sample.

 

3. Sample Preservation

Store samples at -20°C or lower to prevent degradation, avoiding repeated freeze-thaw cycles.

 

Note: Provide details on sample collection and handling.

 

Applications

1. Clinical Diagnosis: Essential for assessing adrenal function and diagnosing disorders such as Addison's disease and Cushing's syndrome.

 

2. Inflammation and Immunity Research: Provides valuable data for investigating cortisone's role in inflammation and immune regulation.

 

3. Drug Research: Supports the study of cortisone-related drug metabolism and pharmacodynamics.

 

4. Hormone Level Evaluation: Assists in evaluating cortisone levels to guide therapeutic interventions.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Cortisone

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into cortisone's extensive physiological roles through top-tier analytical techniques and expert services.