Custom Glycoprotein Analysis Service

Currently, glycoprotein analysis is experiencing rapid development, particularly with the application of mass spectrometry and chromatography techniques. However, there are still shortcomings, such as technical complexity, sample heterogeneity, and a lack of standardized methods, which affect the accuracy and reproducibility of data. Furthermore, existing analysis methods often fail to meet specific research needs, limiting the in-depth understanding of glycosylation functions. Custom glycoprotein analysis addresses these issues by tailoring experimental processes to specific research objectives, thereby improving data accuracy and relevance. Its flexibility allows researchers to tackle complex samples and unique requirements, enabling a deeper exploration of the biological significance of glycoproteins. Additionally, customized services are typically provided by experienced experts, facilitating innovation in research and the translation of findings to clinical applications. Through these advantages, custom glycoprotein analysis offers researchers a more efficient and reliable analytical platform, advancing scientific research progress.

 

Services at MtoZ Biolabs

MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. We utilize an LC-MS-based glycoprotein research platform to provide custom glycoprotein analysis services, ensuring the accuracy of glycoprotein characterization, complex structural analysis, as well as glycoprotein quantification. Our custome glycoprotein analysis services encompass the entire process from sample preparation to data analysis, tailored to meet diverse research needs. Whether you require in-depth analysis of glycoprotein structures or specific identification of functional glycans, we can provide tailored solutions. If you are interested in our custom glycoprotein analysis services, please feel free to contact us at any time. We are committed to providing you with high-quality analytical services and technical support.

 

Analysis Workflow

 



 

Service Advantages

1. Highly Customized Analysis Plans

We design custom glycoprotein analysis workflows tailored to the specific needs of clients, encompassing glycoprotein separation, identification, quantification, and functional analysis to ensure that diverse research and application requirements are met.

 

2. Professional Research Team

MtoZ Biolabs possesses experienced experts and technicians with a strong background in glycoprotein research, capable of providing specialized technical support and scientific advice to optimize experimental design and data interpretation.

 

3. Comprehensive Data Analysis and Reporting

MtoZ Biolabs provides detailed data interpretation and reports, including glycosylation sites, glycan structures, quantitative analysis, and other multidimensional information, helping clients deeply understand research outcomes and support subsequent decision-making.

 

Sample Submission Suggestions

 



If you need more detailed information on other sample submission requirements, feel free to ask!

 

Applications

1. Identification of Biomarkers

There is substantial evidence that protein glycosylation plays a critical role in the onset and progression of diseases. However, direct evidence of protein glycosylation being used to address unmet clinical needs remains limited. Over the past few decades, genomics and proteomics have been extensively explored for biomarker discovery and personalized medicine. Yet, there is still a significant demand for improved diagnostic and stratification strategies for various diseases. Glycosylation, as a post-translational modification of proteins, can affect their structure and function, and it is associated with many common human diseases. Today, many proteins evaluated for plasma levels in clinical practice are glycoproteins. While the glycosylation of these proteins often changes with disease, their glycosylation status has largely been overlooked in clinical settings. As a result, the implementation of glycomic biomarkers in the clinic is still in its early stages. This is largely due to the inherent complexity of protein glycosylation and the analytical techniques required for stable quantification. Mass spectrometry-based workflows are particularly well-suited for the quantitative analysis of glycans and glycoproteins, but a transition from biomedical research environments to clinical laboratories is still needed. Rapidly advancing analytical technologies and well-designed, large-scale glycomics studies specifically targeting these clear unmet clinical needs may accelerate the role of glycomics in clinical trial development in the near future.

 



de, Haan, N. et al. Clin Mass Spectrom. 2020.

 

2. Accelerating Vaccine Development

Glycosylation is a key quality attribute that must be closely monitored in protein therapeutics. Established analytical methods, such as HILIC-Fld for free oligosaccharides and LC-MS for glycopeptides, are suitable for glycoproteins with a limited number of glycosylation sites but are less effective for glycoproteins with multiple glycosylation sites, leading to complex datasets that are time-consuming and challenging to analyze. Researchers have demonstrated that HPAEC-PAD monosaccharide assays can be used to monitor inter-batch variations in the glycosylation of SARS-CoV-2 spike glycoprotein antigens used for vaccine development. The results compare favorably with those obtained from glycan HILIC-Fld and glycopeptide LC-MS analyses. Additionally, researchers have shown that monosaccharide analysis can detect subtle glycosylation differences between batches of the same antigen expressed in the same cell line. Importantly, the execution speed of monosaccharide detection is significantly faster, and the results are quantitative, reproducible, and easy to interpret. Thus, monosaccharide analysis possesses many of the features needed for use in QC environments. Comprehensive analysis of free oligosaccharide post-translational modifications can be highly challenging, and they believe HILIC-Fld and LC-MS are more suited for in-depth analysis of complex glycoproteins. Nevertheless, HPAEC-PAD monosaccharide assays appear to be a better choice for monitoring inter-batch glycosylation of complex glycoproteins. Most importantly, the implementation of these monosaccharide detection strategies can shorten the time required for vaccine development, which is a critical consideration in responding to future pandemics. 

 



Sauvageau, J. et al. Commun Chem. 2023.

 

FAQ

1. How can the detection challenges in glycoprotein analysis caused by glycan heterogeneity be overcome to improve the reliability and reproducibility of results?

To overcome the detection challenges caused by glycan heterogeneity in glycoprotein analysis and improve the reliability and reproducibility of results, the following measures can be taken:

1. Utilizing high-resolution mass spectrometry and multidimensional chromatography techniques to enhance the precision of glycoprotein separation and identification.

2. Standardizing sample preparation procedures to reduce variability during operations.

3. Introducing stable internal standards for quantitative analysis to ensure the reproducibility of results.

4. Incorporating bioinformatics tools to systematically analyze glycosylation patterns, thereby enhancing the accuracy of data interpretation.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Custom Glycoprotein Analysis

5. Mass Spectrometry Image

6. Raw Data