Determination of Antibody Drug Protein Concentration

Antibody drugs are a type of drug that treats diseases through artificially synthesized antibodies. They work by specifically binding to the target molecule to achieve therapeutic purposes. Common types of antibody drugs include monoclonal antibodies, artificially synthesized antibody fragments, immunotoxins, antibody-drug conjugates, etc. Antibody drugs have shown significant therapeutic effects in the treatment of various diseases, such as cancer, autoimmune diseases, inflammatory diseases, immunoregulation, and ophthalmic diseases, etc.

 

In the preparation and use of antibody drugs, protein concentration analysis is a necessary step. The protein concentration can reflect the efficacy and safety of the drug, which is of great significance for drug development, production, and use. Common protein concentration analysis methods include the Bradford method, Lowry method, BCA method, and ultraviolet absorbance spectroscopy.

 

Bradford Method: A dye-binding protein concentration determination method. The advantage of this method is that it is fast, convenient, and not easily disturbed by other substances, but it is sensitive to the type and structure of proteins. The Lowry method is a traditional protein concentration determination method. The Lowry method has high sensitivity, but there are many reaction steps and it is relatively complicated to operate. BCA Method: Also known as bicinchoninic acid method, its principle is similar to the Lowry method, but the BCA method is relatively simple to operate and is not easily disturbed by other substances, but it also has certain sensitivity to the type and structure of proteins. Ultraviolet Absorbance Spectroscopy: The ultraviolet absorbance spectroscopy of proteins is based on the characteristic that the aromatic amino acids in proteins have strong absorption at 280nm. The advantage of this method is that the determination is rapid and does not require any chemical reagents, but it requires knowledge of the composition and sequence of the protein to calculate the exact molar absorption coefficient.

 

Based on the advanced high-resolution chromatographic platform and professional analysis team, MtoZ Biolabs provides efficient and fast protein property analysis service packages. We can quickly and accurately identify the molecular mass, concentration, etc., of proteins, and we can also provide other customized detection solutions according to needs. Free consultation is welcome.