Determination of Monosaccharide Content and Types in Recombinant Protein Vaccines

Recombinant protein vaccine is a type of vaccine that does not contain complete pathogens and is prepared from specific protein antigens produced by heterologous expression systems. Due to their advantages of good safety, strong stability, and relatively low cost, recombinant protein vaccines have been widely favored by researchers in recent years. Currently, recombinant protein vaccines have been widely used in various infectious diseases, such as the prevention of hepatitis B virus (i.e., hepatitis B), tetanus, pertussis, influenza, etc.

 

Monosaccharides are the simplest form of sugars, including glucose, fructose, galactose, etc. In recombinant protein vaccines, monosaccharides often form a part of protein post-translational modification to form glycan structures. These glycan structures have a significant impact on the immunogenicity and stability of the vaccine. During the production process, the content and type of monosaccharides may be affected by many factors, such as the type of host cell, culture conditions, protein folding, and glycan assembly, etc. Therefore, detailed analysis and control of the monosaccharide content and types in recombinant protein vaccines are very important.

 

To analyze the content and types of monosaccharides in recombinant protein vaccines, technologies such as High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) are usually required. HPLC can perform accurate analysis of the type and quantity of monosaccharides. By comparing the retention times of different monosaccharides on the chromatographic column, the types of monosaccharides can be identified. Mass spectrometry can provide more detailed information about the monosaccharides, including the exact mass, structure, and connection method of the monosaccharides. Through mass spectrometry analysis, the types and structures of glycans in recombinant protein vaccines can be comprehensively identified.

 

MtoZ Biolabs provides drug quality research services that comply with global drug regulatory laws and regulations. We use Thermo Fisher's Q Exactive HF mass spectrometry platform combined with Nano-LC to provide a one-stop recombinant protein vaccine monosaccharide content analysis and species identification technology. Just tell us your experimental purpose and send us your samples, MtoZ Biolabs will take care of all the subsequent projects, including sample processing, machine analysis, data analysis, and project reports. Free consultations are welcome.