Determination of the Extinction Coefficient for Recombinant Protein Vaccines

Recombinant protein vaccines are a type of vaccine that does not contain a complete pathogen and are made up of specific protein antigens produced by a heterologous expression system. Common heterologous expression systems include bacteria, mammalian cells, plant cells, and insect cells, and the appropriate heterologous expression system often needs to be selected according to the type of antigen produced. Due to its advantages such as good safety, strong stability, and low cost, recombinant protein vaccines have been widely favored by researchers in recent years. At present, recombinant protein vaccines have been widely used in various infectious diseases, such as the prevention of hepatitis B virus (HBV), tetanus, pertussis, influenza, etc. The research and production of recombinant protein vaccines not only need to understand the biological characteristics of proteins, but also need to ensure their safety and effectiveness through various experimental techniques. Among them, accurately measuring the concentration of recombinant protein vaccines is a very important step, which involves the determination of the extinction coefficient.

 

The extinction coefficient, also known as the molar absorption coefficient or molar extinction coefficient, is a parameter widely used in spectroscopy and molecular physics. The extinction coefficient describes a substance's ability to absorb light of a specific wavelength. The determination of the extinction coefficient is usually carried out by a UV-visible spectrophotometer, which can measure the absorption of a sample to light of a specific wavelength, and then obtain the extinction coefficient. In the determination of the extinction coefficient of recombinant protein vaccines, the absorbance at 280nm of the aromatic amino acids of the protein (such as tyrosine, phenylalanine, and histidine) is usually selected, because these amino acids have strong absorption for this wavelength of light.

 

MtoZ Biolabs provides customers with drug quality research services that comply with global pharmaceutical regulations. Based on our existing spectroscopic experimental platform, we provide you with one-stop recombinant protein vaccine extinction coefficient determination service, you just need to tell us your experimental purpose and send the sample, MtoZ Biolabs is responsible for all subsequent projects, including sample processing, machine analysis, data analysis and project report. Free consultation is welcomed.