Dicoumarol Analysis Service

Dicoumarol is a naturally occurring anticoagulant that was initially isolated from spoiled sweet clover hay and used clinically to prevent thrombosis. It is a coumarin derivative that inhibits the synthesis of vitamin K-dependent clotting factors (such as prothrombin, Factor VII, IX, and X), thereby preventing blood coagulation. Dicoumarol's chemical structure is 1,2-bis(4-hydroxycoumarin) with a molecular formula of C₁₉H₁₂O₆. It features an aromatic ring structure with two coumarin groups linked by a methylene bridge. Dicoumarol is a white to pale yellow crystalline powder, almost insoluble in water but soluble in alcohols and ethers. As a classic anticoagulant, dicoumarol works by inhibiting vitamin K activity, thereby delaying the clotting process. Although its clinical use has been largely replaced by safer drugs, its importance in medicinal chemistry remains significant, particularly in providing structural and mechanistic insights for the development of subsequent anticoagulant drugs.

 



Figure 1. The Structure of Dicoumarol

 

MtoZ Biolabs, a leading provider of proteomics and metabolomics services, proudly offers the advanced Dicoumarol Analysis Service. Leveraging our state-of-the-art high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) platform and a team of expert scientists, we deliver comprehensive and accurate dicoumarol analysis with high sensitivity.

 

Analysis Workflow

 



 

Service Advantages

• High Sensitivity and Accuracy: Our advanced HPLC-MS/MS platform enables us to achieve high sensitivity and accuracy in dicoumarol analysis.
• Comprehensive Analysis: We provide qualitative and quantitative analysis of dicoumarol metabolites, allowing for a thorough understanding of its metabolic pathways.
• Expert Metabolomics Team: Our team of experienced scientists specializes in metabolomics and is dedicated to delivering high-quality results.

 

Applications

• Pharmacology Studies: Dicoumarol analysis can provide valuable insights into the pharmacological effects and mechanisms of action of this compound.
• Drug Development: By analyzing dicoumarol content, we can support drug development by ensuring its safety, efficacy, and quality.
• Natural Product Research: Dicoumarol is found in various natural sources, and our analysis service can help identify and quantify dicoumarol in different samples.
• Mechanistic Studies: Understanding the metabolism of dicoumarol can aid in elucidating its biological activities and potential therapeutic applications.

 

Sample Submission Requirements

1. Sample Types

• We accept a wide range of sample types, including serum, plasma, urine, tissues, and other biological samples.
• For each sample, it is necessary to provide at least 3 replicates with the same condition.

 

2. Sample Volume

• Liquid samples: At least 200 μL
• Animal tissues: At least 200 mg
• Plant tissues: At least 1 g

 

3. Sample Preservation

To maintain sample stability, store samples at -80°C until they are submitted for analysis.

 

Please provide detailed information on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Dicoumarol

4. Raw Data

5. Custom Analysis Report

 

Through our Dicoumarol Analysis Service, we aim to support your research and development endeavors by providing accurate, reliable, and comprehensive analysis of dicoumarol. Contact us today to discuss your project requirements and benefit from our expertise in metabolomics.