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    Dihydroxyacetone Phosphate Analysis Service

      Dihydroxyacetone phosphate (DHAP) is an anion represented by the chemical formula HOCH₂C(O)CH₂OPO₃²⁻. It is a crucial metabolic intermediate in Calvin cycle, glycolysis and lipid metabolism, playing a vital role in numerous biological processes. Accurate, reliable, and sensitive analysis of DHAP is essential for understanding its function in metabolism and disease development. MtoZ Biolabs is pleased to offer a comprehensive DHAP analysis service using our advanced LC-MS/MS platform coupled with our expertise in metabolomics.

       

      1816393330247454720-TheStructureofDihydroxyacetonePhosphate.png

       

      Figure 1. The Structure of Dihydroxyacetone Phosphate

       

      Services at MtoZ Biolabs

      At MtoZ Biolabs, our DHAP analysis service utilizes Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS), a powerful analytical technique that provides superior sensitivity and specificity. It separates complex metabolite mixtures based on their chemical properties, then identifies and quantifies individual metabolites based on their mass-to-charge ratio and fragmentation pattern.

       

      Analysis Workflow

      • Sample Submission: Clients submit samples following our sample submission guidelines.
      • Sample Preparation: We perform extraction, purification, and normalization of the metabolites.
      • LC-MS/MS Analysis: The prepared samples undergo LC-MS/MS analysis.
      • Data Analysis and Report: We perform comprehensive data analysis and provide a detailed report, including data interpretation and recommendations for further studies.

       

      1816392176209874944-WorkflowforDihydroxyacetonePhosphateAnalysisService.png

       

      Applications

      Our DHAP analysis service is applicable to a wide range of research scenarios, including but not limited to:

      • Investigating the role of DHAP in metabolic diseases.
      • Studying the effect of drugs on DHAP levels.
      • Understanding the metabolic pathway of glycolysis and lipid metabolism.

       

      Sample Submission Requirements

      Please ensure your submitted sample surpasses 0.2 g or 0.2 mL.

      Cycle: 2-3 weeks

       

      Deliverables

      1. Experimental Procedures

      2. Relevant Mass Spectrometry Parameters

      3. Detailed Information on Dihydroxyacetone Phosphate Analysis

      4. Mass Spectrometry Images

      5. Raw Data

       

      MtoZ Biolabs is ready to assist you with your DHAP analysis needs. Contact us today to discuss how we can contribute to your research.

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