Disulfide bond

Disulfide bonds are essential covalent bonds in proteins, formed between the sulfur atoms of two cysteine residues. These bonds are crucial for maintaining protein folding, stability, and function. Correct disulfide bond formation is vital for protein structural integrity and biological function, while abnormal disulfide bonds can lead to protein malfunction and disease. Therefore, analyzing the formation and breakage of disulfide bonds is important for understanding protein biology, disease mechanisms, and drug development.

 



Figure 1. Disulfide Bonds in Proteins

 

MtoZ Biolabs offers disulfide bond analysis services using advanced liquid chromatography-tandem mass spectrometry (LC-MS/MS). This technology efficiently and accurately identifies and analyzes disulfide bonds in proteins, providing essential support for scientific research and drug development.

 

Analysis Workflow

 



Service Advantages

• High Sensitivity and Specificity: Utilize high-resolution mass spectrometers to ensure accurate identification and quantification of disulfide bonds.
• Comprehensive Proteome Coverage: Detect and analyze low-abundance disulfide bond proteins, providing a comprehensive connectivity map.
• Dynamic Change Analysis: Monitor dynamic changes in disulfide bond states under different conditions, revealing regulatory mechanisms.
• Efficient Bioinformatics Analysis: Provide detailed disulfide bond information and biological significance through professional data analysis platforms.

 

 

Sample Submission Requirements

 



For detailed sample submission instructions, please consult our technical team.

 

Applications

Protein Folding and Stability Research: Reveal mechanisms of protein folding and stability through disulfide bond analysis.

 

Structural Biology: Identify the location and role of disulfide bonds in the tertiary and quaternary structure of proteins.

 

Disease Mechanism Research: Discover disease-related disulfide bond abnormalities, aiding in disease mechanism research and new drug development.

 

Biopharmaceuticals: Evaluate and optimize disulfide bonds in therapeutic proteins and antibodies, ensuring drug safety and efficacy.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Disulfide Bonds

4. Mass Spectrometry Images

5. Raw Data

 

MtoZ Biolabs, with its advanced technology platforms and extensive project experience, is committed to providing high-quality disulfide bond analysis services to support scientific research and clinical applications. For more information or service inquiries, please contact our technical support team.