Disulfide Bond Localization in Antibody Drugs

Antibody drugs are a type of protein drugs produced by genetic engineering technology, mainly including monoclonal antibodies and antibody-drug conjugates, etc. They have a high degree of specificity and can accurately target and neutralize disease-related molecules. Among these antibody drugs, disulfide bonds are crucial for maintaining the three-dimensional structure and functional stability of the antibodies.

 

In the research and production process of biological drugs, it is essential to accurately detect and control the disulfide bonds in the antibodies. Incorrect disulfide bond pairing may lead to drug failure, or even potentially cause safety risks. Therefore, developing and adopting effective disulfide bond localization methods are crucial for ensuring the quality and safety of antibody drugs. The methods for disulfide bond localization mainly include mass spectrometry, nuclear magnetic resonance, X-ray, and auxiliary chemical methods.

 

Mass spectrometry is a commonly used method for disulfide bond localization, with high sensitivity and resolution. By cutting the antibody protein into multiple peptide fragments with specific enzymes or chemical reagents, then using mass spectrometry to analyze these fragments, the mass of protein fragments can be directly measured, thus determining the position of disulfide bonds. In addition, mass spectrometry can handle relatively large sample volumes and has relatively low requirements for sample purity.

 

MtoZ Biolabs provides customers with drug quality research services that meet global pharmaceutical regulations. Based on years of experimental experience, we provide high-level disulfide bond analysis solutions for antibody drugs. Our solutions include methods to block protein disulfide bond exchange in vitro, sample preparation techniques that preserve the protein’s native structure, and mass spectrometry analysis of disulfide-linked peptides. Additionally, we offer disulfide bond identification through the pLink-SS software, enabling comprehensive analysis of disulfide linkages and free thiols. Disulfide linkages can be confirmed by mass spectrometry to determine the number and positions of disulfide bonds in antibody drugs.After years of experience accumulation, whether it is regular disulfide bonds, overlapping cross-linked disulfide bonds, or mismatched disulfide bonds, MtoZ Biolabs Biotechnology can achieve efficient detection for you.